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Air-displacement pipetting pumps in experimental workflow automation

Application guide

This page explains the air-displacement pipetting pump's position, supporting components and configuration requirements for disposable-tip pipetting on robotic experimental platforms, with links to operating and validation tasks.

Applicable architecture

This page explains how automated operations serve nucleic acid, cell and protein experiments. Parallel channels, resource scheduling and robot-platform integration are covered under Laboratory Automation.

Application guides

Automated Experimental Workflow Guide

Fluidic Overview for Automated Experimental Workflows
Syringe Pumps for Automated Experimental Workflows
Air-displacement pipetting pumps in experimental workflow automation
Disposable-tip pipetting for experiments
Diaphragm pumps in experimental workflow automation
Cleaning recovery
Multiport rotary valves in laboratory workflow automation
Bubble and pressure sensing in experimental workflow automation
Tubing and fittings in laboratory workflow automation

ON THIS PAGE

What do these products do in this instrument?Coordinate air-displacement drive with tips and motionDevelop pipetting sequences for actual liquids and receiving actionsTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageExplore specific operating and validation tasksConfirm product roles in actual operationDoes completed pump movement confirm that the target well received the specified volume?ReferencesRelated guides

01What do these products do in this instrument?

Air-displacement pipetting pumps: tip-based pipetting on robotic platforms

Provide aspiration and dispensing drive on disposable-tip platforms, coordinated with robot motion, liquid level and container position. Establish programs for the actual liquids used in nucleic acid, cell and protein workflows.

SMTP serves disposable-tip air-displacement architectures, with the pump displacing an air column. Tip sealing, liquid type, immersion position and platform motion jointly determine received volume.

02Coordinate air-displacement drive with tips and motion

Air-displacement pipetting pumps move liquid in tips through air-column displacement. Sealing after pickup, tip specification, immersion and platform motion all contribute to aspiration and dispensing. Describe tip-based operations separately from fixed wetted tubing, identifying source well, receiving well and consumable state for each step.

03Develop pipetting sequences for actual liquids and receiving actions

Match aspiration speed, waits, air gaps and dispensing mode to the specific liquid and container. Low remaining volume, foam, droplets and post-tip-change state may affect reception. Schedule tip changes according to contamination-control requirements; when discarding liquid or recovering nucleic-acid eluate, verify destination together with sample identity.

04Translate actual branch conditions into configuration requirements

Air-displacement pipetting pump configuration requirements for laboratory workflow automation
ConditionTo confirm
Tips and sealingProvide tip specifications, pickup/ejection method and sealing checks after pickup.
Liquid and positionRecord liquid type, minimum remaining volume, immersion depth and receiving position.
Results by wellCheck received volume, empty wells or residual liquid, and tip-change and well records.
Operations supported in this instrument
OperationSpecific operating conditions
Disposable-tip pipetting for experimentsDisposable-tip specification and pickup seal, actual liquid class, source volume remaining, immersion depth, working volume and receiving position

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
SMTP air-displacement pipetting pumpAir displacement with disposable tipsValidate tip sealing, liquid type and motion together
  • SMTP air-displacement pipetting pump

06Explore specific operating and validation tasks

Disposable-tip pipetting for experiments

Fixed probes and disposable tips have different washing and consumable requirements. Set actions for the actual reagent, then measure receiving volume and downstream method results separately.

  • Disposable-tip pipetting for experiments

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Disposable-tip pipetting for experimentsMeasure actual receipt at the target vessel, including low source levels, tip pickup and ejection, and contamination across sample sequences. Verify sample or well identity and downstream method results.Tip leaks, aspiration of air, hanging droplets, incorrect immersion or missed tip changes can affect delivery and contamination.

Check liquid delivery, station operations and experimental products together. Workstation throughput does not demonstrate experimental success.

08Does completed pump movement confirm that the target well received the specified volume?

Also check tip sealing, air aspiration, well position and dispensing state, and measure actual reception. The drive-complete signal can assess the operation only together with these records.

General platform content is maintained under Laboratory Automation; life science pages retain experimental outcome and contamination requirements.

References

View references (1)
  1. [1]FOREACH SMTP air-displacement pipetting pump: configurations and specifications

Related guides

  • Back to experimental workflow automation fluidics
  • Disposable-tip pipetting for experiments

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SMTP air-displacement pipetting pump; representative configuration. Select capacity, materials and interfaces for the application.

SMTP air-displacement pipetting pump

Related Technical Articles

What is a programmable automatic air-displacement pipetting pump?

A programmable automatic air-displacement pipetting pump is a liquid-handling component whose aspiration, dispense, speeds and sequence are set through control commands. This article focuses on air-displacement modules that use disposable tips in OEM instruments, with FOREACH SMTP2 as an example of operation, monitoring and selection limits.

2026-09-28View Details

What Is Pipetting? Manual and Automated Workflows Compared

Pipetting is the transfer of a specified volume of liquid from one location to another. Examples include moving 100 µL from a sample tube into a reaction vessel, or adding sample and diluent in a defined ratio.

2026-09-29View Details

Discuss fluidic configurations for life science automation

Provide a fluid diagram, the actual liquids and pressures in each state. Tip sealing, liquid type and motion require joint validation. Compare suitable configurations against the validation objectives described here.

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