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Home/Applications/Life science/Experimental workflow automation
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Diaphragm pumps in experimental workflow automation

Application guide

This page explains diaphragm pump positions, supporting components and configuration requirements for shared washing or replenishment and suitable gas-containing wash-station waste aspiration in automated experiments, with links to operating and validation tasks.

Applicable architecture

This page explains how automated operations serve nucleic acid, cell and protein experiments. Parallel channels, resource scheduling and robot-platform integration are covered under Laboratory Automation.

Application guides

Automated Experimental Workflow Guide

Fluidic Overview for Automated Experimental Workflows
Syringe Pumps for Automated Experimental Workflows
Air-displacement pipetting pumps in experimental workflow automation
Disposable-tip pipetting for experiments
Diaphragm pumps in experimental workflow automation
Cleaning recovery
Multiport rotary valves in laboratory workflow automation
Bubble and pressure sensing in experimental workflow automation
Tubing and fittings in laboratory workflow automation

ON THIS PAGE

What do these products do in this instrument?Define liquid-supply and aerated-aspiration branches separatelyMatch supply/removal capacity to washing-station and container conditionsTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageExplore specific operating and validation tasksConfirm product roles in actual operationDo similar nominal supply- and waste-pump flow rates make a matched pair?ReferencesRelated guides

01What do these products do in this instrument?

Liquid diaphragm pumps: shared washing or replenishment supply

Supply actual shared wash stations or reservoir-replenishment branches in coordination with station occupancy and peak demand. After workstation actions, check receipt and washing recovery at each station.

Compare DPL30, DPL60 and DPL30H by actual supply load. Wash-head, valve, tubing and container resistance jointly determine installed flow; check the supply window and receiving position together.

Gas-liquid diaphragm pumps: suitable air-containing wash-station drainage

Drain wash stations using suitable gas-containing aspiration or vessel-vacuum architectures, coordinated with incoming wash liquid and waste-container conditions. Shared waste capacity must cover the workstations allowed to run concurrently.

DPGL800 handles gas or gas–liquid aspiration permitted by its specific configuration. Check inlet gas content, foam, peak inflow and container vacuum; use a separately suitable solution for continuous liquid-only transfer.

02Define liquid-supply and aerated-aspiration branches separately

Suitable liquid diaphragm-pump configurations supply wash heads, stations or working vessels. Gas–liquid diaphragm pumps serve permitted aerated aspiration or related container-vacuum architectures. Describe actual inlet medium and gas–liquid state first, then determine pump position, incoming source and discharge destination.

03Match supply/removal capacity to washing-station and container conditions

Installed flow depends jointly on pump, tubing, valves, wash head and container; free flow does not directly represent station delivery. Evaluate supply by effective coverage and available time, and waste removal by peak inflow, lift, permitted residual liquid and full-container state. Check the complete wash cycle when supply and aspiration must be coordinated.

04Translate actual branch conditions into configuration requirements

Diaphragm-pump configuration requirements for laboratory workflow automation
ConditionTo confirm
Inlet mediumDistinguish liquid-only flow, intermittent gas entrainment and foam, and confirm the aspiration modes permitted by the configuration.
Tubing and containersProvide suction lift, outlet load, tubing and waste-container conditions.
Actual supply and removalMeasure incoming liquid, residue and emptying at the station; check handling after full level, shortage or blockage.
Operations supported in this instrument
OperationSpecific operating conditions
Cleaning recoveryWash-fluid formulation, volume/time, spray/aspiration geometry, air/foam and waste capacity

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
DPL30 liquid diaphragm pumpLiquid supply or auxiliary circulationSelect by installed resistance, actual wash or working fluid and task window
DPL60 liquid diaphragm pumpLiquid supply or auxiliary circulationJudge by peak demand and installed operating point, not free flow alone
DPL30H high-backpressure liquid diaphragm pumpLiquid branches requiring higher backpressure capabilityCalculate pressure budget and actual flow first, then confirm interfaces and materials
DPGL800 gas–liquid diaphragm pumpSuitable gas–liquid aspiration or container-vacuum branchesConfirm inlet medium and waste architecture; not for continuous liquid-only transfer (100% liquid)
  • DPL30 liquid diaphragm pump
  • DPL60 liquid diaphragm pump
  • DPL30H high-backpressure liquid diaphragm pump
  • DPGL800 gas–liquid diaphragm pump

06Explore specific operating and validation tasks

Cleaning recovery

Nucleic-acid contamination, protein adsorption and cell residue may require different cleaning solutions. Evaluate cleaning with method-relevant blanks or subsequent samples, rather than probe appearance alone.

  • Cleaning recovery

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Cleaning recoveryConfirm supply and peak waste-removal capacity under installed resistance; measure residual liquid, carryover or assay-specified recoveryInsufficient supply, foam, dry aspiration and a full waste container disrupt the cleaning cycle

Check liquid delivery, station operations and experimental products together. Workstation throughput does not demonstrate experimental success.

08Do similar nominal supply- and waste-pump flow rates make a matched pair?

Compare peak inflow, effective aspiration and permissible accumulation in the actual installation. The branches may handle different media and operating windows; validate station recovery over the complete sequence.

General platform content is maintained under Laboratory Automation; life science pages retain experimental outcome and contamination requirements.

References

View references (4)
  1. [1]FOREACH DPL30 liquid diaphragm pump: configurations and specifications
  2. [2]FOREACH DPL60 liquid diaphragm pump: configurations and specifications
  3. [3]FOREACH DPL30H high-backpressure liquid diaphragm pump: configurations and specifications
  4. [4]FOREACH DPGL800 gas–liquid diaphragm pump: configurations and specifications

Related guides

  • Back to experimental workflow automation fluidics
  • Cleaning recovery

Related Products

DPL30 liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPL30 liquid diaphragm pump

DPL60 liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPL60 liquid diaphragm pump

DPL30H high-backpressure liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPL30H high-backpressure liquid diaphragm pump

DPGL800 gas–liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPGL800 gas–liquid diaphragm pump

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Selecting a Self-Priming Miniature Liquid Diaphragm Pump: Lift, Priming Time and Dry or Wet Starts

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What is a programmable automatic air-displacement pipetting pump?

A programmable automatic air-displacement pipetting pump is a liquid-handling component whose aspiration, dispense, speeds and sequence are set through control commands. This article focuses on air-displacement modules that use disposable tips in OEM instruments, with FOREACH SMTP2 as an example of operation, monitoring and selection limits.

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Discuss fluidic configurations for life science automation

Provide a fluidic diagram, actual liquids and pressures in each operating state. Select using installed resistance, actual wash or working liquid and task duration. Assess peak demand and the installed operating point rather than free flow alone. Calculate the pressure budget and actual flow before confirming interfaces and materials. Identify the inlet medium and waste-removal architecture; the gas-liquid option is not for continuous transfer of 100% liquid. Compare suitable configurations against this guide's validation objectives.

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