Where tip-based laboratory pipetting occurs in the instrument
This task covers disposable-tip air-displacement operations in laboratory workflow automation. The pipetting pump drives liquid in the tip while the platform coordinates sources, receiving locations and consumables. Check tip pickup sealing, the actual liquid and sample identity before setting aspiration, dispensing and motion programs.
Fixed probes and disposable tips have different washing and consumable requirements. Set actions for the actual reagent, then measure receiving volume and downstream method results separately.
Product responsibilities in this operation
The air-displacement pump drives liquid inside the tip; the platform manages tips, wells and movement. Associate liquid destinations and consumable states with every operation.
For this automated experimental task, define disposable-tip specifications and pickup sealing, actual liquid classes, remaining source volume, immersion depth, working volume and receiving position.
Organize the sequence by actual architecture
- This task uses disposable-tip air displacement. Confirm the actual liquid, target dose, source and receiving wells for the nucleic acid, cell or protein experiment. See syringe-pump applications for fixed-needle drive.
- Check sealing after tip pickup, and match immersion position, aspiration/dispensing and platform motion to the current liquid program.
- Check actual delivery at the designated vessel. Change and eject tips according to experimental contamination controls, maintaining the association between consumables, wells and sample identity.
- Check downstream target recovery, cell condition or method blanks separately. If delivery is partial, record the current state before deciding whether to complete or terminate the operation.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Disposable-tip specification and pickup seal, actual liquid class, source volume remaining, immersion depth, working volume and receiving position |
| Acceptance target | Measure actual receipt at the target vessel, including low source levels, tip pickup and ejection, and contamination across sample sequences. Verify sample or well identity and downstream method results. |
| Fault localization | Tip leaks, aspiration of air, hanging droplets, incorrect immersion or missed tip changes can affect delivery and contamination. |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Validate operation results at the receiver
Measure actual volume received in the target vessel, covering low remaining volume, tip pickup/disposal and contamination across sample sequences. Verify sample or well identity and subsequent method results.
Check liquid delivery, station operations and experimental products together. Workstation throughput does not demonstrate experimental success.
Locate abnormalities and restore the next operation
Tip air leaks, air aspiration, retained droplets, incorrect immersion or missed tip changes can affect delivery and contamination status.
Check the volume already delivered and the destination vessel first. Handle the tip and affected sample according to the experimental procedure, then verify readiness before the next action.
General platform content is maintained under Laboratory Automation; life science pages retain experimental outcome and contamination requirements.