Where washing and return to service occur in the instrument
Design washing around sources of molecular contamination, cell residue or protein adsorption. Evaluate wash supply and air-containing waste removal separately, then confirm readiness with the next sample or a blank.
Nucleic-acid contamination, protein adsorption and cell residue may require different cleaning solutions. Evaluate cleaning with method-relevant blanks or subsequent samples, rather than probe appearance alone.
Product responsibilities in this operation
Diaphragm pumps perform suitable liquid supply or aerated aspiration in this task. Confirm inlet phase, branch location and outlet destination separately, then check each branch's operating point.
Compare DPL30, DPL60 and DPL30H by actual supply load. Wash-head, valve, tubing and container resistance jointly determine installed flow; check the supply window and receiving position together.
DPGL800 handles gas or gas–liquid aspiration permitted by its specific configuration. Check inlet gas content, foam, peak inflow and container vacuum; use a separately suitable solution for continuous liquid-only transfer.
For this automated experimental task, define wash-liquid formulation, volume and duration, spray or aspiration geometry, air or foam conditions, and waste capacity.
Organize the sequence by actual architecture
- Identify reusable probes, fixed paths and wash stations on the actual platform. Select wash liquid and cleaning locations for nucleic-acid contamination, protein adsorption or cell residue. Handle disposable tips according to tip-change rules.
- Use the supply branch to achieve the required coverage and contact. Check station occupancy, actual incoming liquid and the washing window.
- Choose drainage for the actual liquid-only or gas-containing inlet condition. Check peak liquid accumulation and waste-container status.
- Confirm recovery using method-relevant blanks or actual results from the next sample. Keep separate records for delivered volume and experimental contamination.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Wash-fluid formulation, volume/time, spray/aspiration geometry, air/foam and waste capacity |
| Acceptance target | Confirm that wash fluid reaches the reusable surfaces affected by nucleic-acid contamination, protein adsorption or cell residue. Check contact, residual liquid and drainage with the actual station load and inlet phase. |
| Fault localization | Missed surfaces, unsuitable contact conditions or inadequate drainage can leave molecular or cellular residue. A visually clean probe or completed wash command does not establish readiness for the next experiment. |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| DPL30 liquid diaphragm pump | Liquid supply or auxiliary circulation | Select by installed resistance, actual wash or working fluid and task window |
| DPL60 liquid diaphragm pump | Liquid supply or auxiliary circulation | Judge by peak demand and installed operating point, not free flow alone |
| DPL30H high-backpressure liquid diaphragm pump | Liquid branches requiring higher backpressure capability | Calculate pressure budget and actual flow first, then confirm interfaces and materials |
| DPGL800 gas–liquid diaphragm pump | Suitable gas–liquid aspiration or container-vacuum branches | Confirm inlet medium and waste architecture; not for continuous liquid-only transfer (100% liquid) |
Validate operation results at the receiver
Confirm that wash fluid reaches the reusable surfaces affected by nucleic-acid contamination, protein adsorption or cell residue. Check contact, residual liquid and drainage with the actual station load and inlet phase.
Use the experiment-relevant blank or next sample to judge return to service. Record contamination results separately from delivered wash volume and workstation timing.
Locate abnormalities and restore the next operation
Missed surfaces, unsuitable contact conditions or inadequate drainage can leave molecular or cellular residue. A visually clean probe or completed wash command does not establish readiness for the next experiment.
Hold the affected reusable path and samples, resolve coverage or waste-removal faults, and repeat the permitted cleaning procedure. Verify the relevant contamination blank or next-sample result before releasing the path for experimental work.
General platform content is maintained under Laboratory Automation; life science pages retain experimental outcome and contamination requirements.
References
View references (4)
- [1]FOREACH DPL30 liquid diaphragm pump: configurations and specifications
- [2]FOREACH DPL60 liquid diaphragm pump: configurations and specifications
- [3]FOREACH DPL30H high-backpressure liquid diaphragm pump: configurations and specifications
- [4]FOREACH DPGL800 gas–liquid diaphragm pump: configurations and specifications