What do these products do in this instrument?
Air-displacement pipetting pumps: tip-based environmental sample transfer
Drive aliquoting and solution preparation in disposable-tip architectures, coordinated with liquid class, mixing and container identity. Record original container, sampling time and receiver, and check actual receipt and between-sample contamination.
SMTP serves disposable-tip air-displacement architectures, with the pump displacing an air column. Tip sealing, liquid type, immersion position and platform motion jointly determine received volume.
Coordinate air-displacement drive with tips and motion
Air-displacement pipetting pumps move liquid in tips through air-column displacement. Sealing after pickup, tip specification, immersion and platform motion all contribute to aspiration and dispensing. Describe tip-based operations separately from fixed wetted tubing, identifying source well, receiving well and consumable state for each step.
Develop pipetting sequences for actual liquids and receiving actions
Match aspiration speed, waits, air gaps and dispensing mode to the specific liquid and container. Low remaining volume, foam, droplets and post-tip-change state may affect reception. Schedule tip changes according to contamination-control requirements; when discarding liquid or recovering nucleic-acid eluate, verify destination together with sample identity.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Tips and sealing | Provide tip specifications, pickup/ejection method and sealing checks after pickup. |
| Liquid and position | Record liquid type, minimum remaining volume, immersion depth and receiving position. |
| Results by well | Check received volume, empty wells or residual liquid, and tip-change and well records. |
| Operation | Specific operating conditions |
|---|---|
| Tip-based environmental sample aliquoting and transfer | Disposable-tip specification and pickup seal, actual liquid class, source volume remaining, immersion depth, working volume and receiving position |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Explore specific operating and validation tasks
Tip-based environmental sample aliquoting and transfer
Record the original vessel, preservation state and destination. Particle-containing samples may differ when aliquoted at different times; include mixing and transfer order in the program.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Tip-based environmental sample aliquoting and transfer | Measure actual receipt at the target vessel, including low source levels, tip pickup and ejection, and contamination across sample sequences. Verify sample or well identity and downstream method results. | Tip leaks, aspiration of air, hanging droplets, incorrect immersion or missed tip changes can affect delivery and contamination. |
Evaluate blanks, recovery, aliquot representativeness and restart according to the method.
Does completed pump movement confirm that the target well received the specified volume?
Also check tip sealing, air aspiration, well position and dispensing state, and measure actual reception. The drive-complete signal can assess the operation only together with these records.
Dilution metering can share general engineering methods, but field preservation, particles and analyte loss require separate checks.