What do these products do in this instrument?
Air-displacement pipetting pumps: tip-based protein sample preparation
Transfer samples, buffers or reaction formulations in disposable-tip workstations, using programs matched to the liquid and container. For low concentrations or volumes, check received volume, analyte recovery and blanks together.
SMTP serves disposable-tip air-displacement architectures, with the pump displacing an air column. Tip sealing, liquid type, immersion position and platform motion jointly determine received volume.
Coordinate air-displacement drive with tips and motion
Air-displacement pipetting pumps move liquid in tips through air-column displacement. Sealing after pickup, tip specification, immersion and platform motion all contribute to aspiration and dispensing. Describe tip-based operations separately from fixed wetted tubing, identifying source well, receiving well and consumable state for each step.
Develop pipetting sequences for actual liquids and receiving actions
Match aspiration speed, waits, air gaps and dispensing mode to the specific liquid and container. Low remaining volume, foam, droplets and post-tip-change state may affect reception. Schedule tip changes according to contamination-control requirements; when discarding liquid or recovering the protein-containing fraction, verify destination together with sample identity.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Tips and sealing | Provide tip specifications, pickup/ejection method and sealing checks after pickup. |
| Liquid and position | Record liquid type, minimum remaining volume, immersion depth and receiving position. |
| Results by well | Check received volume, empty wells or residual liquid, and tip-change and well records. |
| Operation | Specific operating conditions |
|---|---|
| Sample transfer | Actual sample volume, remaining source volume, probe-tip height, aspiration/dispensing speed and system liquid |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Related operations and architecture comparisons
Sample transfer
Pass protein samples through the actual tubing and vessels and compare with reference handling. Water calibration assesses volume; protein recovery assesses materials and workflow. Record them separately.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Sample transfer | Measure delivered volume, protein recovery, aggregation or method response, and carryover between samples together. | Material adsorption, aggregation, hanging droplets or residue can reduce recovery even when delivered volume meets requirements. |
Compare recovery and blanks before and after the complete path, and check the response after buffer switching.
Does completed pump movement confirm that the target well received the specified volume?
Also check tip sealing, air aspiration, well position and dispensing state, and measure actual reception. The drive-complete signal can assess the operation only together with these records.
A material name alone does not establish low adsorption. Confirm sample preparation, loading and pressurized separation branches separately.