What do these products do in this instrument?
Tubing and Fittings: Protein-Contact Surfaces in the Flow Path
Connect pumps, valves, sources and receivers. Include fittings and flexible/rigid tubing in recovery assessment with the actual protein. Material names and water-volume tests support initial screening; complete-path results establish suitability.
Tubing and fittings together form sealed connections and the actual transfer path. Check formulation, inner/outer diameters, length, tube-end positioning and port geometry, including added volume in residue and arrival delay.
Design tubing and fittings as a complete wetted path
Tubing, fittings and ports jointly determine sealing, transfer volume and medium-contact coverage. Materials and dimensions must suit formulation, operating conditions and installation. Include tubing length and interface volume in priming, shared residue and arrival delay. After connection changes, recheck delivery and cleaning through the actual path.
Calculate connection volume and post-maintenance recovery
Shared volume affects liquid arrival and flushing; interface gaps may retain the previous composition. Record length, actual bore and tube insertion position. After disassembly, reprime and check seals, then compare first-cycle delivery, blanks or recovered product to confirm path recovery.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Materials and assembly | Provide formulation, tubing inner/outer diameters, length, fitting structure and port fit. |
| Volume and load | Calculate shared and replacement-component added volumes; measure actual flow and response. |
| Maintenance recovery | Record disassembly locations and added volume; recheck priming, sealing and first-cycle received results. |
| Operation | Specific operating conditions |
|---|---|
| Sample transfer | Actual sample volume, remaining source volume, probe-tip height, aspiration/dispensing speed and system liquid |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| Tubing materials and dimensions | Connection paths and shared volume | Bore, length, load and actual formulation jointly determine materials and dimensions |
| Rigid-tubing fittings | Tube-end positioning and sealed connections | Check tube outer diameter, threads, seal structure and port-bottom geometry |
Related operations and architecture comparisons
Sample transfer
Pass protein samples through the actual tubing and vessels and compare with reference handling. Water calibration assesses volume; protein recovery assesses materials and workflow. Record them separately.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Sample transfer | Measure delivered volume, protein recovery, aggregation or method response, and carryover between samples together. | Material adsorption, aggregation, hanging droplets or residue can reduce recovery even when delivered volume meets requirements. |
Compare recovery and blanks before and after the complete path, and check the response after buffer switching.
Does a fitting that tightens prove the tubing assembly is suitable?
Also confirm port geometry, tube-end positioning and seal fit. Check leakage, hold-up and performance after repeated maintenance under actual conditions; thread engagement alone is insufficient.
A material name alone does not establish low adsorption. Confirm sample preparation, loading and pressurized separation branches separately.