What do these products do in this instrument?
Air-Displacement Pipetting Pumps: Disposable-Tip Transfer and Preparation
Transfer samples or liquid phases with disposable tips, accounting for liquid class, interfaces and receiving positions. Identify the target-containing phase and define retained and discarded fractions before evaluating recovery and phase carryover.
SMTP serves disposable-tip air-displacement architectures, with the pump displacing an air column. Tip sealing, liquid type, immersion position and platform motion jointly determine received volume.
Coordinate air-displacement drive with tips and motion
Air-displacement pipetting pumps move liquid in tips through air-column displacement. Sealing after pickup, tip specification, immersion and platform motion all contribute to aspiration and dispensing. Describe tip-based operations separately from fixed wetted tubing, identifying source well, receiving well and consumable state for each step.
Develop pipetting sequences for actual liquids and receiving actions
Match aspiration speed, waits, air gaps and dispensing mode to the specific liquid and container. Low remaining volume, foam, droplets and post-tip-change state may affect reception. Schedule tip changes according to contamination-control requirements; when discarding liquid or recovering nucleic-acid eluate, verify destination together with sample identity.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Tips and sealing | Provide tip specifications, pickup/ejection method and sealing checks after pickup. |
| Liquid and position | Record liquid type, minimum remaining volume, immersion depth and receiving position. |
| Results by well | Check received volume, empty wells or residual liquid, and tip-change and well records. |
| Operation | Specific operating conditions |
|---|---|
| Tip-based transfer of extraction liquids and target phases | Phase or solid phase to retain, waste and eluate destinations, aspiration height, allowable residue and analyte |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Explore specific operating and validation tasks
Tip-based transfer of extraction liquids and target phases
Loading, washing and elution send liquids to different destinations. Record phase carryover, product loss and vessel residue to assess aspiration position and switching timing.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Tip-based transfer of extraction liquids and target phases | Measure target recovery, phase carryover, blanks and residue | Incorrect aspiration position, phase carryover or surface adsorption may cause product loss. |
Evaluate ratio, mixing, blanks and target recovery at the receiving position.
Does completed pump movement confirm that the target well received the specified volume?
Also check tip sealing, air aspiration, well position and dispensing state, and measure actual reception. The drive-complete signal can assess the operation only together with these records.
Filtration and extraction can change sample composition. Mechanical protection does not replace method-recovery validation.