Where tip-based extract and target-phase transfer occur
Distinguish loading, washing, elution and phase transfer for the extraction method. Define phase state, pressure and product destinations, then evaluate target recovery, phase carryover and residue.
Loading, washing and elution send liquids to different destinations. Record phase carryover, product loss and vessel residue to assess aspiration position and switching timing.
Product responsibilities in this operation
The air-displacement pump drives liquid inside the tip; the platform manages tips, wells and movement. Associate liquid destinations and consumable states with every operation.
For this analytical sample preparation task, confirm the retained phase or solid phase, waste and eluate destinations, aspiration height, permitted residual liquid and analyte.
Organize the sequence by actual architecture
- For the specific extraction method, identify the phase containing the analyte, liquids to retain or discard, and receiving vessels. This page focuses on liquid transfers that tips can perform.
- Check tip compatibility with the actual solvent, sealing and container interfaces. Set aspiration height, speed and residual-liquid control to avoid mixing the target and unwanted phases.
- Transfer the appropriate liquid after method-specified separation or elution. Retain sample identity and destination records, and check phase carryover, vessel residue and consumables needing replacement.
- Assess the complete process using analyte recovery, phase carryover, blanks and residue. Operations requiring pressure through a fixed bed or another pressurized path need a suitable device configuration; air-displacement motion is not a direct substitute.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Phase or solid phase to retain, waste and eluate destinations, aspiration height, allowable residue and analyte |
| Acceptance target | For each loading, washing, elution or phase-transfer step, confirm the retained liquid and its destination. Measure analyte recovery, unwanted-phase carryover, vessel residue and blanks using the actual solvent and tip geometry. |
| Fault localization | An incorrect aspiration interface, emulsion or retained droplet can transfer the wrong phase or lose product. Adsorption can reduce analyte recovery despite acceptable liquid volume. |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Validate operation results at the receiver
For each loading, washing, elution or phase-transfer step, confirm the retained liquid and its destination. Measure analyte recovery, unwanted-phase carryover, vessel residue and blanks using the actual solvent and tip geometry.
Compare transfer at the intended phase interface and sample volume with the complete extraction result. A full receiving vessel does not establish that the correct phase or target fraction was recovered.
Locate abnormalities and restore the next operation
An incorrect aspiration interface, emulsion or retained droplet can transfer the wrong phase or lose product. Adsorption can reduce analyte recovery despite acceptable liquid volume.
Preserve the identities of source, waste and product vessels and determine which phase was already transferred. Re-establish separation and replace affected consumables as permitted by the method before resuming; do not repeat a discard step on material of uncertain identity.
This task covers tip-based liquid transfers. Pressurized extraction through a fixed bed or other loaded path requires separately suitable equipment and method validation.