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Home/Applications/Synthetic biology/Biofoundry Construct Development and Screening
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Multiport rotary valves in Biofoundry construction and screening

Application guide

Explore multiport rotary-valve placement, coordination and configuration for source and destination selection in Biofoundry construction and screening, with links to operating and validation tasks.

Application guides

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ON THIS PAGE

What do these products do in this instrument?Define connections using the instrument's port-state diagramInclude renewal of shared segments after switching in the sequenceTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageExplore specific operating and validation tasksConfirm product roles in actual operationDo more valve ports mean more channels can supply liquid simultaneously?ReferencesRelated guides

01What do these products do in this instrument?

Multiport Rotary Valves: Multiple-Source and Destination Selection

In multi-source fixed fluidics, connect the reagent for the current construction task in coordination with metering and resource interlocks. Record source, destination well and valve position together, with defined flushing after shared-path switching.

MRV3 selects sources or destinations and requires a port-state diagram and shared-volume budget. Port count indicates routing options; confirm pressure in each state and independent simultaneous supply requirements separately.

02Define connections using the instrument's port-state diagram

This page covers multiport rotary valves. Mark every port, current source, receiving destination and branch requiring isolation, and check connections during aspiration, delivery, cleaning, maintenance and power loss. Valve selection must correspond to conditions permitting the drive action.

03Include renewal of shared segments after switching in the sequence

After source switching, shared tubing may retain the previous composition. Record volume and arrival delay from the new source to the receiver, and measure residue and recovery for the actual worst-case switching combination. Confirm port positioning and arrival of new liquid separately.

04Translate actual branch conditions into configuration requirements

Multiport rotary valve configuration requirements for Biofoundry construction and screening
ConditionTo confirm
Connections and pressureProvide port diagrams and pressure conditions for all operating, cleaning and shutdown states.
Shared volumeIdentify residue locations after source changes, flushing volumes and discharge destinations.
Control and isolationCheck valve-position feedback, pump actions, shutoff leakage and permitted power-off states.
Operations supported in this instrument
OperationSpecific operating conditions
Path schedulingTask state, sample or vessel identity, concurrent resources, interlocks and restart

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
MRV3 multiport rotary valveMultiple-source selectionCompare 10-, 16- and 24-port options and port diagrams; verify high-pressure suitability separately
  • MRV3 multiport rotary valve

06Explore specific operating and validation tasks

Path scheduling

Confirm valve position together with reagent source and target well. Restart programs should preserve sample identity and completed-step records to prevent duplicate additions.

  • Path scheduling

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Path schedulingTest concurrent operation, limiting states and complete fault recovery; verify that identities, cumulative quantities and records match actual actionsResource conflicts, communication loss or mismatched identities can cause delivery to the wrong destination or repeated dosing.

Assess per-well delivery, identity traceability and construct-development or screening results together.

08Do more valve ports mean more channels can supply liquid simultaneously?

Check port connection structure and drive configuration. The number of selectable sources, independent paths and simultaneous-operation capability are different conditions, determined by actual connections in each state.

High throughput and multiple channels do not establish valid experimental results. See laboratory automation for shared workstation architecture.

References

View references (1)
  1. [1]FOREACH MRV3 multiport rotary valve: configurations and specifications

Related guides

  • Back to Biofoundry construction and screening fluidics
  • Path scheduling

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MRV3 multiport rotary valve; representative configuration. Select capacity, materials and interfaces for the application.

MRV3 multiport rotary valve

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Discuss a biofoundry fluidic configuration

Provide a fluid diagram, the actual liquids and pressures in each state. Compare 10-, 16- and 24-port configurations against the port map, and assess high-pressure suitability separately. Compare suitable configurations against the validation objectives described here.

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