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Home/Applications/Synthetic biology/Biofoundry Construct Development and Screening
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Biofoundry construction and screening: path scheduling

Application guide

This guide covers path scheduling in Biofoundry construction and screening: component roles, action sequence, operating conditions and restart requirements. Identify the architecture before comparing configurations.

Application guides

Biofoundry construction and screening application guide

Biofoundry construction and screening fluidics overview
Syringe Pumps for Biofoundry Construct Development and Screening
Biological-reagent aspiration and dispensing
Air-Displacement Pipetting Pumps for Biofoundry Construct Development and Screening
Disposable-tip pipetting of construct samplesPer-well delivery for screening plates
Diaphragm Pumps for Biofoundry Construct Development and Screening
Multiport rotary valves in Biofoundry construction and screening
Path scheduling
Bubble and Pressure Monitoring for Biofoundry Construct Development and Screening
Tubing and Fittings for Biofoundry Construct Development and Screening

ON THIS PAGE

Where path scheduling occurs in the instrumentProduct responsibilities in this operationOrganize the sequence by actual architectureDefine operating conditions for this taskCompare product configurations by the roles on this pageValidate operation results at the receiverLocate abnormalities and restore the next operationReferencesRelated guides

01Where path scheduling occurs in the instrument

Bind valve positions, reagent sources and target plate positions in the control system, including shared-resource interlocks. After an anomaly, retain sample identity and completed steps to prevent misdelivery or duplicate dispensing.

Confirm valve position together with reagent source and target well. Restart programs should preserve sample identity and completed-step records to prevent duplicate additions.

02Product responsibilities in this operation

Valves control the current source or destination; the associated drive performs metering or transfer. Port connections, actual positioning and shared-segment renewal must match the operation.

For this Biofoundry construction and screening task, confirm task state, sample/vessel identity, concurrent resources, interlocks and recovery.

03Organize the sequence by actual architecture

  1. In fixed-path configurations, map reagent sources, valve ports, target plate positions and construction tasks to each other. Identify shared resources and permitted occupancy.
  2. Confirm resources and valve position before drive operation. On source changes, refresh shared sections and record actual receipt.
  3. After an anomaly, retain reagent, well and completed-step records. Handle undelivered, partially delivered and completed states separately to prevent misdelivery and duplicate dispensing.

04Define operating conditions for this task

Conditions, results and abnormality records for this operation
To defineDetails
Actual operating conditionsTask state, sample or vessel identity, concurrent resources, interlocks and restart
Acceptance targetTest concurrent operation, limiting states and complete fault recovery; verify that identities, cumulative quantities and records match actual actions
Fault localizationResource conflicts, communication loss or mismatched identities can cause delivery to the wrong destination or repeated dosing.

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
MRV3 multiport rotary valveMultiple-source selectionCompare 10-, 16- and 24-port options and port diagrams; verify high-pressure suitability separately
  • MRV3 multiport rotary valve

06Validate operation results at the receiver

Cover concurrency, boundary states and complete recovery after faults. Check that identity, cumulative volume and records match actual operations.

Assess per-well delivery, identity traceability and construct-development or screening results together.

07Locate abnormalities and restore the next operation

Resource conflicts, communication interruptions or identity mismatches can cause misdelivery or duplicate dispensing.

Stop the affected branches and recover using execution records.

High throughput and multiple channels do not establish valid experimental results. See laboratory automation for shared workstation architecture.

References

View references (1)
  1. [1]FOREACH MRV3 multiport rotary valve: configurations and specifications

Related guides

  • Back to Biofoundry construction and screening fluidics
  • Multiport rotary valves in Biofoundry construction and screening

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MRV3 multiport rotary valve; representative configuration. Select capacity, materials and interfaces for the application.

MRV3 multiport rotary valve

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Discuss a biofoundry fluidic configuration

Provide a fluid diagram, the actual liquids and pressures in each state. Compare 10-, 16- and 24-port configurations against the port map, and assess high-pressure suitability separately. Compare suitable configurations against the validation objectives described here.

Contact an engineer