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Home/Applications/Synthetic biology/Biofoundry Construct Development and Screening
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Tubing and Fittings for Biofoundry Construct Development and Screening

Application guide

This page explains the positions, supporting components and configuration requirements of tubing and fittings connecting fixed stations and shared paths in biofoundry construct development and screening, with links to operating and validation tasks.

Application guides

Biofoundry construction and screening application guide

Biofoundry construction and screening fluidics overview
Syringe Pumps for Biofoundry Construct Development and Screening
Biological-reagent aspiration and dispensing
Air-Displacement Pipetting Pumps for Biofoundry Construct Development and Screening
Disposable-tip pipetting of construct samplesPer-well delivery for screening plates
Diaphragm Pumps for Biofoundry Construct Development and Screening
Multiport rotary valves in Biofoundry construction and screening
Path scheduling
Bubble and Pressure Monitoring for Biofoundry Construct Development and Screening
Tubing and Fittings for Biofoundry Construct Development and Screening

ON THIS PAGE

What do these products do in this instrument?Design tubing and fittings as a complete wetted pathCalculate connection volume and post-maintenance recoveryTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageRelated operations and architecture comparisonsConfirm product roles in actual operationDoes a fitting that tightens prove the tubing assembly is suitable?ReferencesRelated guides

01What do these products do in this instrument?

Tubing and fittings: fixed-station and shared-path connections

Connect reagent sources, drives, wash stations and receivers, identifying independent and shared wetted sections. Assess sample changes, maintenance and restart together with cross-contamination and experimental results.

Tubing and fittings together form sealed connections and the actual transfer path. Check formulation, inner/outer diameters, length, tube-end positioning and port geometry, including added volume in residue and arrival delay.

02Design tubing and fittings as a complete wetted path

Tubing, fittings and ports jointly determine sealing, transfer volume and medium-contact coverage. Materials and dimensions must suit formulation, operating conditions and installation. Include tubing length and interface volume in priming, shared residue and arrival delay. After connection changes, recheck delivery and cleaning through the actual path.

03Calculate connection volume and post-maintenance recovery

Shared volume affects liquid arrival and flushing; interface gaps may retain the previous composition. Record length, actual bore and tube insertion position. After disassembly, reprime and check seals, then compare first-cycle delivery, blanks or recovered product to confirm path recovery.

04Translate actual branch conditions into configuration requirements

Tubing and fitting configuration for Biofoundry construction and screening
ConditionTo confirm
Materials and assemblyProvide formulation, tubing inner/outer diameters, length, fitting structure and port fit.
Volume and loadCalculate shared and replacement-component added volumes; measure actual flow and response.
Maintenance recoveryRecord disassembly locations and added volume; recheck priming, sealing and first-cycle received results.
Operations supported in this instrument
OperationSpecific operating conditions
Biological-reagent aspiration and dispensingDose per operation, total cycle volume, replenishment/bottle-change interval, backpressure and receiving geometry

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
Tubing materials and dimensionsConnection paths and shared volumeBore, length, load and actual formulation jointly determine materials and dimensions
Rigid-tubing fittingsTube-end positioning and sealed connectionsCheck tube outer diameter, threads, seal structure and port-bottom geometry
  • Tubing materials and dimensions
  • Rigid-tubing fittings

06Related operations and architecture comparisons

Biological-reagent aspiration and dispensing

Set standby and storage requirements for enzyme solutions, media and inducers from the actual workflow. Check the first dose with the real liquids and record waiting time.

  • Biological-reagent aspiration and dispensing

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Biological-reagent aspiration and dispensingUse actual media to check first, middle and last doses and delivery after bottle changes; confirm received volume and timing rather than substituting commanded steps for volumeEmpty sources, bubbles, nozzle droplets and backpressure changes affect first or last doses

Assess per-well delivery, identity traceability and construct-development or screening results together.

08Does a fitting that tightens prove the tubing assembly is suitable?

Also confirm port geometry, tube-end positioning and seal fit. Check leakage, hold-up and performance after repeated maintenance under actual conditions; thread engagement alone is insufficient.

High throughput and multiple channels do not establish valid experimental results. See laboratory automation for shared workstation architecture.

References

View references (2)
  1. [1]FOREACH tubing materials and dimensions: configurations and specifications
  2. [2]FOREACH rigid-tubing fittings: configurations and specifications

Related guides

  • Back to Biofoundry construction and screening fluidics

Related Products

Tubing materials and dimensions; representative configuration. Select capacity, materials and interfaces for the application.

Tubing materials and dimensions

Rigid-tubing fittings; representative configuration. Select capacity, materials and interfaces for the application.

Rigid-tubing fittings

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What is flow resistance calculation for? Using the Foreach calculator in fluid-path design and selection

The free flow rate of the pump meets the requirements. After installing the pipelines, valves and joints, the liquid supply at the end is insufficient. Flow resistance calculations can break the problem down into comparable data: what pressure differential is required at the target flow rate, where does the pressure loss mainly occur, and what happens if the pipe diameter, pipe length, component Cv or medium viscosity is changed. This article combines the actual function of the Foreach flow resistance calculator with a recalculable fluid circuit case to explain how to use the calculation results for design, selection, troubleshooting and prototype verification.

2026-09-11View Details

Why Does Actual Flow Change So Much When the Tubing Size Changes on the Same Miniature Diaphragm Pump?

Even when the pump is unchanged, installed flow can change because of tubing inner diameter, length, bends, and the minimum bore of fittings. When flow falls after a tubing change, the effective response is neither to blame the pump first nor to enlarge every tube indiscriminately. Measure inlet pressure, outlet pressure, and accumulated flow together to determine where the added restriction actually occurs.

2026-08-24View Details

What is a programmable automatic air-displacement pipetting pump?

A programmable automatic air-displacement pipetting pump is a liquid-handling component whose aspiration, dispense, speeds and sequence are set through control commands. This article focuses on air-displacement modules that use disposable tips in OEM instruments, with FOREACH SMTP2 as an example of operation, monitoring and selection limits.

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Discuss a biofoundry fluidic configuration

Provide a fluid diagram, the actual liquids and pressures in each state. Bore size, length, load and actual formulation jointly determine material and dimensional choices. Check tubing OD, threads, sealing structure and port-bottom geometry. Compare suitable configurations against the validation objectives described here.

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