Where disposable-tip construct-sample pipetting fits in the instrument
This task covers disposable-tip air displacement in biofoundry construct development and screening. The pump drives liquid in the tip while the platform coordinates sources, receivers and consumable motions. Check pickup sealing, actual liquids and sample identity before setting aspiration, dispensing and motion programs.
Set aspiration, dispensing and tip changes for the liquid type. After an anomaly, confirm delivery status first. Correct individual doses can still result in an incorrect batch if delivered to the wrong wells.
Product responsibilities in this operation
The air-displacement pump drives liquid inside the tip; the platform manages tips, wells and movement. Associate liquid destinations and consumable states with every operation.
For biofoundry construct development and screening, define disposable-tip specifications and pickup sealing, actual liquid class, source volume, immersion depth, working volume and receiver position.
Organize the sequence by actual architecture
- This task uses disposable tips. Associate construct or strain identity, reagent source, source well and destination well with the operation record.
- Check tip sealing, immersion position and aspiration/dispensing actions for actual liquid types and doses. Keep platform motion aligned with the current well.
- Check actual delivery and change tips according to contamination controls. Record each well as not started, partially completed or complete.
- Assess operations using cross-contamination checks and corresponding construction or screening results. Do not repeat an entire plate after interruption without first reconciling its status.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Disposable-tip specification and pickup seal, actual liquid class, source volume remaining, immersion depth, working volume and receiving position |
| Acceptance target | Measure actual receipt at the target vessel, including low source levels, tip pickup and ejection, and contamination across sample sequences. Verify sample or well identity and downstream method results. |
| Fault localization | Tip leaks, aspiration of air, hanging droplets, incorrect immersion or missed tip changes can affect delivery and contamination. |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Validate operation results at the receiver
Measure actual volume received in the target vessel, covering low remaining volume, tip pickup/disposal and contamination across sample sequences. Verify sample or well identity and subsequent method results.
Assess per-well delivery, identity traceability and construct-development or screening results together.
Locate abnormalities and restore the next operation
Tip air leaks, air aspiration, retained droplets, incorrect immersion or missed tip changes can affect delivery and contamination status.
Check the volume already delivered and the destination vessel first. Handle the tip and affected sample according to the experimental procedure, then verify readiness before the next action.
High throughput and multiple channels do not establish valid experimental results. See laboratory automation for shared workstation architecture.