Where tip-based washing and nucleic-acid eluate transfer occur in the instrument
In architectures that pipette liquid while retaining solid phase, washing removes supernatant and post-elution transfer recovers nucleic-acid eluate; define aspiration height, permitted residue and destinations separately. With magnetic-rod bead transfer, focus on solid-phase transfer, reagent wells and eluate reception, identifying which replenishment or transfer steps actually require liquid-drive components.
Evaluate received eluate volume, target recovery, residual wash fluid and downstream inhibition risk separately. Volume, extraction results and contamination blanks are different evidence and must all be interpreted with the complete method.
Product responsibilities in this operation
The air-displacement pump drives liquid inside the tip; the platform manages tips, wells and movement. Associate liquid destinations and consumable states with every operation.
For this task in molecular diagnostics, confirm solid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements.
Organize the sequence by actual architecture
- Confirm a tip-based liquid-transfer architecture retaining solid phase, marking discarded wash liquid, post-elution nucleic-acid eluate and the next reaction well. Organize magnetic-rod architectures separately by actual solid-phase motion.
- Set aspiration height by solid-phase position, send liquid for disposal to waste and add wash fluid according to the method. Tip changes and consumable order follow contamination-control requirements.
- After specified elution and solid-phase separation, transfer the nucleic-acid eluate to retain into its designated receiver, checking recovered volume and residual wash fluid.
- Verify eluate identity, target recovery, negative blanks and downstream usability. After interruption, establish transferred volume before continuing to avoid repeated disposal or duplicate delivery.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Solid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements |
| Acceptance target | During tip-based washing, check that the required solid phase remains while discarded liquid reaches waste. During post-elution transfer, measure eluate in the identified receiver and evaluate remaining wash liquid and target recovery. |
| Fault localization | Incorrect aspiration height can discard solid phase or retain unwanted wash liquid. A wrong destination can lose eluate, while duplicate aspiration after interruption can further reduce recovery. |
Further technical reading
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Validate operation results at the receiver
During tip-based washing, check that the required solid phase remains while discarded liquid reaches waste. During post-elution transfer, measure eluate in the identified receiver and evaluate remaining wash liquid and target recovery.
Use negative blanks and the downstream method to assess contamination and inhibition separately from recovered volume. Confirm the correct sample and destination well before the next setup step.
Locate abnormalities and restore the next operation
Incorrect aspiration height can discard solid phase or retain unwanted wash liquid. A wrong destination can lose eluate, while duplicate aspiration after interruption can further reduce recovery.
Hold the source and receiving wells and determine which wash, discard or eluate-transfer step completed. Establish remaining solid phase, liquid and transferred volume before deciding how the method permits continuation; verify tip state and sample identity before the next action.
Tip-based liquid transfer and magnetic-rod bead transfer use different movements. Neither pump completion nor correct eluate volume alone establishes extraction quality.