Where fixed-path lysis and binding reagent dispensing occurs in the instrument
Manage doses, materials and mixing separately for lysis solution, binding solution and beads. Choose fixed paths or disposable tips by sample contamination, formulation and actual workload, paying attention to first doses after idle and particle condition.
Confirm materials and action conditions separately for lysis and binding solutions. Record sample identity, target well and completed delivery steps; one water sequence cannot cover all working liquids.
Product responsibilities in this operation
Syringe drive and valve positions jointly organize aspiration, holding and dispensing. Record working volume, remaining inventory and replenishment timing according to the sequence.
For this operation, confirm: Lysis, binding and particle-containing formulations; source valves; fixed-path materials; priming destination; working dose; target well; mixing and processing sequence.
Organize the sequence by actual architecture
- Confirm that the architecture requires fixed fluidic supply, listing lysis, binding and other reagent formulations, sample positions and target containers.
- Prime and aspirate with the syringe at the corresponding source-valve position, directing shared residue to its specified destination.
- Deliver reagents in assay order, coordinated with separately defined mixing and processing times. The pump delivers liquid; that action alone cannot establish completion of lysis or binding.
- Check actual received volume, subsequent nucleic-acid recovery and contamination blanks separately, retaining source and sample identity records for recovery decisions.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Lysis, binding and particle-containing formulations; source valves; fixed-path materials; priming destination; working dose; target well; mixing and processing sequence |
| Acceptance target | Measure each lysis or binding reagent at the intended receiving well using its actual formulation and source path. Include first doses after standby or replenishment and verify the order of completed additions. |
| Fault localization | An empty source, air entry, shared reagent residue or wrong receiving well can alter extraction conditions. Replaying a completed pump command may duplicate a reagent addition without restoring an interrupted extraction. |
Further technical reading
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| HLD rotary-valve syringe pump | Aspiration, holding and dispensing with a rotary valve | Check source/outlet valve positions, capacity budget and replenishment timing |
| HMD solenoid-valve syringe pump | Metered aspiration and dispensing with solenoid valves | Check open/closed and power-off states, working volume and replenishment cycle |
Validate operation results at the receiver
Measure each lysis or binding reagent at the intended receiving well using its actual formulation and source path. Include first doses after standby or replenishment and verify the order of completed additions.
Assess subsequent nucleic-acid recovery and contamination blanks with the complete extraction method. Keep these results separate from volume checks and from the mixing or processing time required for lysis and binding.
Locate abnormalities and restore the next operation
An empty source, air entry, shared reagent residue or wrong receiving well can alter extraction conditions. Replaying a completed pump command may duplicate a reagent addition without restoring an interrupted extraction.
Hold the affected wells and reconcile sample identity, reagent source and volume already received. Restore the source and priming path, then follow the extraction method for partially processed samples before resuming reagent delivery.
This task applies to fixed-path reagent supply. A completed delivery does not prove lysis or binding, and magnetic-rod or disposable-tip architectures require their own handling sequence.