What do these products do in this instrument?
Air-displacement pipetting pumps: tip-based sample and reaction-mixture transfer
Provide air-displacement drive for samples, reagents or nucleic-acid eluate in disposable-tip architectures. Coordinate tip pickup/ejection, motion and well records, with separate sequences for extraction and downstream setup.
SMTP serves disposable-tip air-displacement architectures, with the pump displacing an air column. Tip sealing, liquid type, immersion position and platform motion jointly determine received volume.
Coordinate air-displacement drive with tips and motion
Air-displacement pipetting pumps move liquid in tips through air-column displacement. Sealing after pickup, tip specification, immersion and platform motion all contribute to aspiration and dispensing. Describe tip-based operations separately from fixed wetted tubing, identifying source well, receiving well and consumable state for each step.
Further technical reading
Develop pipetting sequences for actual liquids and receiving actions
Match aspiration speed, waits, air gaps and dispensing mode to the specific liquid and container. Low remaining volume, foam, droplets and post-tip-change state may affect reception. Schedule tip changes according to contamination-control requirements; when discarding liquid or recovering nucleic-acid eluate, verify destination together with sample identity.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Tips and sealing | Provide tip specifications, pickup/ejection method and sealing checks after pickup. |
| Liquid and position | Record liquid type, minimum remaining volume, immersion depth and receiving position. |
| Results by well | Check received volume, empty wells or residual liquid, and tip-change and well records. |
| Operation | Specific operating conditions |
|---|---|
| Tip-based washing and nucleic-acid eluate transfer | Solid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Explore specific operating and validation tasks
Tip-based washing and nucleic-acid eluate transfer
Aspiration speed, solid-phase position and residue affect the next step. Washing removes supernatant while elution recovers nucleic-acid eluate, requiring different heights and destinations; validate with recovery and blanks.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Tip-based washing and nucleic-acid eluate transfer | Evaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery together | Insufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery |
Check recovered volume, extracted nucleic-acid usability and between-sample contamination, maintaining separation of wash fluid and waste.
Does completed pump movement confirm that the target well received the specified volume?
Also check tip sealing, air aspiration, well position and dispensing state, and measure actual reception. The drive-complete signal can assess the operation only together with these records.
Valve switching or low dead volume alone does not prove contamination isolation for extracted nucleic acid and amplification setup.