What do these products do in this instrument?
Air-displacement pipetting pumps: disposable-tip operation
Drive liquid within the tip through air-column displacement, coordinated with tip sealing, immersion position and motion. Multiple dispenses from one aspiration also require air-gap and retained-volume accounting.
SMTP serves disposable-tip air-displacement architectures, with the pump displacing an air column. Tip sealing, liquid type, immersion position and platform motion jointly determine received volume.
Coordinate air-displacement drive with tips and motion
Air-displacement pipetting pumps move liquid in tips through air-column displacement. Sealing after pickup, tip specification, immersion and platform motion all contribute to aspiration and dispensing. Describe tip-based operations separately from fixed wetted tubing, identifying source well, receiving well and consumable state for each step.
Develop pipetting sequences for actual liquids and receiving actions
Match aspiration speed, waits, air gaps and dispensing mode to the specific liquid and container. Low remaining volume, foam, droplets and post-tip-change state may affect reception. Schedule tip changes according to contamination-control requirements; when discarding liquid or recovering nucleic-acid eluate, verify destination together with sample identity.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Tips and sealing | Provide tip specifications, pickup/ejection method and sealing checks after pickup. |
| Liquid and position | Record liquid type, minimum remaining volume, immersion depth and receiving position. |
| Results by well | Check received volume, empty wells or residual liquid, and tip-change and well records. |
| Operation | Specific operating conditions |
|---|---|
| Single transfers with disposable tips | Disposable-tip specification and pickup seal, actual liquid class, source volume remaining, immersion depth, working volume and receiving position |
| Multiple dispenses from one aspiration | Dose per operation, total cycle volume, replenishment/bottle-change interval, backpressure and receiving geometry |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Explore specific operating and validation tasks
Single transfers with disposable tips
Viscosity, volatility and surface tension can alter air-displacement performance. Calibrate with the actual tips and containers, then assess low source volumes and high-speed operation.
Multiple dispenses from one aspiration
Nominal capacity minus retained volume determines the usable dispensing range. Record every dose, comparing first, middle and last doses and the first dose after replenishment, rather than measuring the average alone.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Single transfers with disposable tips | Measure actual receipt at the target vessel, including low source levels, tip pickup and ejection, and contamination across sample sequences. Verify sample or well identity and downstream method results. | Tip leaks, aspiration of air, hanging droplets, incorrect immersion or missed tip changes can affect delivery and contamination. |
| Multiple dispenses from one aspiration | Use actual media to check first, middle and last doses and delivery after bottle changes; confirm received volume and timing rather than substituting commanded steps for volume | Empty sources, bubbles, nozzle droplets and backpressure changes affect first or last doses |
Measure delivery with actual liquids, tips and well positions, including idle periods, refills and multiple dispensing.
Does completed pump movement confirm that the target well received the specified volume?
Also check tip sealing, air aspiration, well position and dispensing state, and measure actual reception. The drive-complete signal can assess the operation only together with these records.
Nominal capacity and volume per step do not establish the minimum reliable pipetting volume. Disposable tips and reusable probes require different cleaning strategies.