Where multiple dispensing from one aspiration fits in the instrument
Calculate each delivery, air gap, first and last dose, and retained volume for the actual program. Identify every channel and receiver. Nominal capacity is not all available as dispensable reagent. Compare first, middle, last and first-after-refill doses rather than the average alone.
Product responsibilities in this operation
The air-displacement pump drives liquid inside the tip; the platform manages tips, wells and movement. Associate liquid destinations and consumable states with every operation.
For this automated pipetting task, define individual dose, total cycle volume, refill or bottle-change interval, backpressure and receiver geometry.
Organize the sequence by actual architecture
- List dose count, individual volume, retained volume and destination-well sequence. Determine the usable total dispense volume within the tip's permitted capacity.
- Check pickup sealing and source liquid level, then aspirate once using the actual liquid class. Associate the available volume with the destination-vessel list.
- Dispense well by well, recording actual receipt and remaining volume for each dose. Compare first, middle and last doses separately; a cycle average cannot replace individual-dose results.
- When aspirating again or changing tips, handle sources and consumables according to the contamination-control strategy and check the next cycle's first dose. After an interruption, identify completed and partially filled wells before choosing where to resume.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Dose per operation, total cycle volume, replenishment/bottle-change interval, backpressure and receiving geometry |
| Acceptance target | Use actual media to check first, middle and last doses and delivery after bottle changes; confirm received volume and timing rather than substituting commanded steps for volume |
| Fault localization | Empty sources, bubbles, nozzle droplets and backpressure changes affect first or last doses |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Validate operation results at the receiver
Use actual media to check first, middle and last doses and delivery after bottle changes; confirm received volume and timing, not commanded steps as a proxy for volume.
Measure delivery with actual liquids, tips and well positions, including idle periods, refills and multiple dispensing.
Locate abnormalities and restore the next operation
Empty sources, bubbles, nozzle droplets and backpressure changes may affect first or last doses.
Stop affected steps, validate recovery after priming and avoid duplicate dispensing.
Nominal capacity and volume per step do not establish the minimum reliable pipetting volume. Disposable tips and reusable probes require different cleaning strategies.