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Home/Applications/Laboratory automation/Automated Pipetting
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Sampling Probes for Automated Pipetting

Application guide

This page explains sampling-needle location, coordination and configuration requirements for fixed-needle interfaces at vessels in automated pipetting, with links to relevant operating and validation tasks.

Applicable architecture

This page compares fixed-probe liquid-displacement and disposable-tip air-displacement configurations. Probe or consumable shape alone does not establish the drive architecture. Confirm the actual displacement method and wetted components from the equipment design.[2]

Application guides

Automated pipetting application guide

Automated pipetting fluidics overview
Piston pumps in automated pipetting
Syringe pumps in automated pipetting
Air-Displacement Pipetting Pumps for Automated Pipetting
Single transfers with disposable tipsMultiple dispenses from one aspiration
Sampling Probes for Automated Pipetting
Washing or tip change
Bubble and Pressure Monitoring for Automated Pipetting
Tubing and fittings in automated pipetting

ON THIS PAGE

What do these products do in this instrument?Match component geometry to actual containersEvaluate interfaces by receiving or cleaning resultsTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageExplore specific operating and validation tasksConfirm product roles in actual operationCan sampling and wash probes be replaced based on similar appearance alone?ReferencesRelated guides

01What do these products do in this instrument?

Sampling probes: fixed-probe interface at the vessel

In fixed-probe configurations, connect source liquid and target container while coordinating drive, liquid level and motion. Probe position, hanging droplets and internal/external washing affect the actual pipetting sequence.

Custom sampling probes connect source containers to receiving positions. Diameter, tip, surface and interface depend on sample and container. Tip geometry must work with metering drive and motion sequences.

02Match component geometry to actual containers

Sampling probes must fit container openings, bottoms, minimum levels and motion strokes. Record actual contact surfaces and permitted positions before defining tips, interfaces and motion space; appearance alone is insufficient.

03Evaluate interfaces by receiving or cleaning results

At the source, check reachable positions and low remaining volume; at the receiver, check actual volume, droplets or permitted residue. Test wetted-surface maintenance and recovery for the next container during consecutive operation. Reconfirm position and delivery after changing probes, tubing length or containers.

04Translate actual branch conditions into configuration requirements

Sampling Probe Configuration for Automated Pipetting
ConditionTo confirm
Container and positionProvide opening, bottom, minimum level, positioning error and available motion space.
Liquid contact and surfacesSpecify sample or reagent, residue requirements and internal/external probe cleaning.
Actual functionCheck delivery, coverage, residue, solid-phase retention or mixing results according to the task.
Operations supported in this instrument
OperationSpecific operating conditions
Washing or tip changeFixed-probe or tip architecture, wetted surfaces, tip-change rules and contamination targets

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
Custom sampling probeSampling interface between source container and fluidic pathCompare custom geometry by minimum liquid level, probe-tip clearance and actual sample
  • Custom sampling probe

06Explore specific operating and validation tasks

Washing or tip change

For reusable probes, manage internal and external wetted surfaces. For disposable tips, manage pickup, changes and disposal. Use sample sequences and blanks to determine whether the strategy is adequate.

  • Washing or tip change

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Washing or tip changeFor fixed needles, check washing and blank recovery. For tip-based architectures, check pickup sealing, tip replacement and contamination across sample sequences.Insufficient wash coverage, a missed tip change or a failed seal may affect the next sample

Measure delivery with actual liquids, tips and well positions, including idle periods, refills and multiple dispensing.

08Can sampling and wash probes be replaced based on similar appearance alone?

Confirm tip, channels, interfaces and operating positions separately, then validate each probe's aspiration/dispensing or cleaning results. Similar appearance does not imply equal wetted volume, coverage or residual-liquid levels.

Nominal capacity and volume per step do not establish the minimum reliable pipetting volume. Disposable tips and reusable probes require different cleaning strategies.

References

View references (2)
  1. [1]FOREACH custom sampling probes: configurations and specifications
  2. [2]Hamilton air-displacement pipetting channels, tips, monitoring and motion technologies

Related guides

  • Back to the automated pipetting fluidics overview
  • Washing or tip change

Related Products

Custom sampling probe; representative configuration. Select capacity, materials and interfaces for the application.

Custom sampling probe

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Contact an engineer