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Home/Applications/Laboratory automation/Automated Pipetting
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Bubble and Pressure Monitoring for Automated Pipetting

Application guide

Explore sensing locations, coordination and configuration for associating fluid-state information with pipetting stages, with links to operating and validation tasks.

Applicable architecture

This page compares fixed-probe liquid-displacement and disposable-tip air-displacement configurations. Probe or consumable shape alone does not establish the drive architecture. Confirm the actual displacement method and wetted components from the equipment design.[3]

Application guides

Automated pipetting application guide

Automated pipetting fluidics overview
Piston pumps in automated pipetting
Syringe pumps in automated pipetting
Air-Displacement Pipetting Pumps for Automated Pipetting
Single transfers with disposable tipsMultiple dispenses from one aspiration
Sampling Probes for Automated Pipetting
Washing or tip change
Bubble and Pressure Monitoring for Automated Pipetting
Tubing and fittings in automated pipetting

ON THIS PAGE

What do these products do in this instrument?Match sensing locations to specific fluidic problemsConnect signals, action windows and recovery conditionsTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageRelated operations and architecture comparisonsConfirm product roles in actual operationCan dispensing be repeated immediately after bubbles or abnormal pressure are detected?ReferencesRelated guides

01What do these products do in this instrument?

Bubble and Pressure Monitoring: Signals Linked to Pipetting Stages

Detect bubbles or pressure changes at permitted tubing locations. The controller records source, target container and completed doses. Check tip or probe sealing and actual delivery independently.

ABD provides bubble or liquid-state signals for defined tubing; PDM5 provides pressure information at a defined location. Configure module, position and action window separately; the controller associates affected samples or tasks.

02Match sensing locations to specific fluidic problems

Bubble modules report bubble or liquid state in defined tube segments; pressure modules report pressure at defined positions. Before selecting mounting points, define the phase and abnormality to observe: aspiration, delivery, source depletion or branch-load changes. Associate sensor positions with affected samples, cuvettes or stations.

03Connect signals, action windows and recovery conditions

The same signal may mean different things at different valve positions or operating phases. Record normal ranges and faults with actual liquids, tubing and sequences. The controller combines action timing, confirmed delivery status, volume records and sample identity to pause, review or recover; one pressure change cannot directly identify a blockage location or specific cause. Distinguish measured, estimated and unknown volumes; completion of a command does not prove actual liquid delivery.

04Translate actual branch conditions into configuration requirements

Bubble and Pressure Monitoring Configuration for Automated Pipetting
ConditionTo confirm
Installation conditionsSpecify tubing dimensions, liquid, sensing location and branch to observe.
Timing and baselineRecord valve positions, pump actions, normal changes and fault-detection windows.
Abnormality handlingAssociate affected containers with confirmed delivery status and volume records; define conditions for review, continuation or re-execution. Distinguish measured, estimated and unknown volumes; completion of a command does not prove actual liquid delivery.
Operations supported in this instrument
OperationSpecific operating conditions
Multiple dispenses from one aspirationDose per operation, total cycle volume, replenishment/bottle-change interval, backpressure and receiving geometry

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
ABD bubble-detection moduleBubble or liquid-state identification in defined tubingValidate detection and false alarms for tubing material, dimensions, medium and action window
PDM5 pressure-detection modulePressure-state monitoring at a defined fluidic locationConfirm range and wetted configuration by model; validate normal baseline and abnormalities separately
  • ABD bubble-detection module
  • PDM5 pressure-detection module

06Related operations and architecture comparisons

Multiple dispenses from one aspiration

Nominal capacity minus retained volume determines the usable dispensing range. Record every dose, comparing first, middle and last doses and the first dose after replenishment, rather than measuring the average alone.

  • Multiple dispenses from one aspiration

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Multiple dispenses from one aspirationUse actual media to check first, middle and last doses and delivery after bottle changes; confirm received volume and timing rather than substituting commanded steps for volumeEmpty sources, bubbles, nozzle droplets and backpressure changes affect first or last doses

Measure delivery with actual liquids, tips and well positions, including idle periods, refills and multiple dispensing.

08Can dispensing be repeated immediately after bubbles or abnormal pressure are detected?

First establish whether partial delivery has occurred and which container is affected. Immediate repetition may double-dose it. Act on the actual state and recheck the first cycle after recovery.

Nominal capacity and volume per step do not establish the minimum reliable pipetting volume. Disposable tips and reusable probes require different cleaning strategies.

References

View references (3)
  1. [1]FOREACH ABD bubble-detection module: configurations and specifications
  2. [2]FOREACH PDM5 pressure-detection module: configurations and specifications
  3. [3]Hamilton air-displacement pipetting channels, tips, monitoring and motion technologies

Related guides

  • Back to the automated pipetting fluidics overview

Related Products

ABD bubble-detection module; representative configuration. Select capacity, materials and interfaces for the application.

ABD bubble-detection module

PDM5 pressure-detection module; representative configuration. Select capacity, materials and interfaces for the application.

PDM5 pressure-detection module

Related Technical Articles

Why Do Air Bubbles Delay or Bias Piston Pump Dispensing?

A completed piston stroke does not prove that the intended liquid volume reached the vessel. Bubble compression temporarily stores displacement; expansion can then cause a trailing dispense or affect the next cycle. Locate where bubbles first appear and compare synchronized pressure and individual dispense masses before and after priming.

2026-09-10View Details

What is flow resistance calculation for? Using the Foreach calculator in fluid-path design and selection

The free flow rate of the pump meets the requirements. After installing the pipelines, valves and joints, the liquid supply at the end is insufficient. Flow resistance calculations can break the problem down into comparable data: what pressure differential is required at the target flow rate, where does the pressure loss mainly occur, and what happens if the pipe diameter, pipe length, component Cv or medium viscosity is changed. This article combines the actual function of the Foreach flow resistance calculator with a recalculable fluid circuit case to explain how to use the calculation results for design, selection, troubleshooting and prototype verification.

2026-09-11View Details

What Is Pipetting? Manual and Automated Workflows Compared

Pipetting is the transfer of a specified volume of liquid from one location to another. Examples include moving 100 µL from a sample tube into a reaction vessel, or adding sample and diluent in a defined ratio.

2026-09-29View Details

Discuss fluidic configurations for automated pipetting

Provide a fluid-path diagram, actual liquids and pressures in each state. Validate detection and false alarms for the tubing, dimensions, media and action window. Confirm range and wetted configuration by model, then test normal baselines and faults separately against the guide's validation objectives.

Contact an engineer