What do these products do in this instrument?
Tubing and fittings: fixed-path sealing and shared volume
Connect system liquid, pumps, valves and the probe in fixed-probe architectures, maintaining sealing and priming. For disposable tips, assess the actual air channel and seal design. Document wetted components separately for each architecture.
Tubing and fittings together form sealed connections and the actual transfer path. Check formulation, inner/outer diameters, length, tube-end positioning and port geometry, including added volume in residue and arrival delay.
Design tubing and fittings as a complete wetted path
Tubing, fittings and ports jointly determine sealing, transfer volume and medium-contact coverage. Materials and dimensions must suit formulation, operating conditions and installation. Include tubing length and interface volume in priming, shared residue and arrival delay. After connection changes, recheck delivery and cleaning through the actual path.
Calculate connection volume and post-maintenance recovery
Shared volume affects liquid arrival and flushing; interface gaps may retain the previous composition. Record length, actual bore and tube insertion position. After disassembly, reprime and check seals, then compare first-cycle delivery, blanks or recovered product to confirm path recovery.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Materials and assembly | Provide formulation, tubing inner/outer diameters, length, fitting structure and port fit. |
| Volume and load | Calculate shared and replacement-component added volumes; measure actual flow and response. |
| Maintenance recovery | Record disassembly locations and added volume; recheck priming, sealing and first-cycle received results. |
| Operation | Specific operating conditions |
|---|---|
| Multiple dispenses from one aspiration | Dose per operation, total cycle volume, replenishment/bottle-change interval, backpressure and receiving geometry |
| Washing or tip change | Fixed-probe or tip architecture, wetted surfaces, tip-change rules and contamination targets |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| Tubing materials and dimensions | Connection paths and shared volume | Bore, length, load and actual formulation jointly determine materials and dimensions |
| Rigid-tubing fittings | Tube-end positioning and sealed connections | Check tube outer diameter, threads, seal structure and port-bottom geometry |
Related operations and architecture comparisons
Multiple dispenses from one aspiration
Nominal capacity minus retained volume determines the usable dispensing range. Record every dose, comparing first, middle and last doses and the first dose after replenishment, rather than measuring the average alone.
Washing or tip change
For reusable probes, manage internal and external wetted surfaces. For disposable tips, manage pickup, changes and disposal. Use sample sequences and blanks to determine whether the strategy is adequate.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Multiple dispenses from one aspiration | Use actual media to check first, middle and last doses and delivery after bottle changes; confirm received volume and timing rather than substituting commanded steps for volume | Empty sources, bubbles, nozzle droplets and backpressure changes affect first or last doses |
| Washing or tip change | For fixed needles, check washing and blank recovery. For tip-based architectures, check pickup sealing, tip replacement and contamination across sample sequences. | Insufficient wash coverage, a missed tip change or a failed seal may affect the next sample |
Measure delivery with actual liquids, tips and well positions, including idle periods, refills and multiple dispensing.
Does a fitting that tightens prove the tubing assembly is suitable?
Also confirm port geometry, tube-end positioning and seal fit. Check leakage, hold-up and performance after repeated maintenance under actual conditions; thread engagement alone is insufficient.
Nominal capacity and volume per step do not establish the minimum reliable pipetting volume. Disposable tips and reusable probes require different cleaning strategies.