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Home/Applications/Life science/Cell culture and transfer
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Diaphragm pumps in cell culture and transfer

Application guide

This page explains diaphragm-pump location, coordination and configuration requirements for auxiliary culture-medium supply and washing in cell culture and transfer, with links to relevant operating and validation tasks.

Application guides

Cell culture and transfer application guide

Cell culture and transfer fluidics overview
Syringe Pumps for Cell Culture and Transfer
Suspension transfer
Valveless metering pumps in cell culture and transfer
Repeated and continuous medium supply
Diaphragm pumps in cell culture and transfer
Tubing pinch-off solutions in cell culture and transfer
Bubble and pressure sensing in cell culture and transfer
Tubing and fittings in cell culture and transfer

ON THIS PAGE

What do these products do in this instrument?Assign liquid supply to a defined usage branchCoordinate supply windows with downstream receiving conditionsTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageConfirm product roles in actual operationDo similar nominal supply- and waste-pump flow rates make a matched pair?ReferencesRelated guides

01What do these products do in this instrument?

Liquid Diaphragm Pumps: Auxiliary Medium Supply and Cleaning

Transfer working liquid in bulk-supply or washing branches that actually exist in the instrument, coordinated with reservoirs and valve paths. Confirm medium delivery separately from cell-suspension transfer, and assess wetted configurations under actual culture conditions.

Compare DPL30, DPL60 and DPL30H by actual supply load. Wash-head, valve, tubing and container resistance jointly determine installed flow; check the supply window and receiving position together.

02Assign liquid supply to a defined usage branch

This page compares liquid diaphragm-pump configurations for suitable supply paths. Define source, point of use, working liquid and cycle time, then measure liquid arriving at the use point under actual load. Cleaning effectiveness also depends on formulation, coverage and contact time.

03Coordinate supply windows with downstream receiving conditions

Tubing, valves and end-use resistance change installed flow. Match supply to permitted container levels or cleaning inflow. Downstream drainage uses its own extraction architecture; check its media and container protection separately rather than inferring them from supply-pump capacity.

04Translate actual branch conditions into configuration requirements

Diaphragm-pump configuration requirements for cell culture and transfer
ConditionTo confirm
Inlet mediumDistinguish liquid-only flow, intermittent gas entrainment and foam, and confirm the aspiration modes permitted by the configuration.
Tubing and containersProvide suction lift, outlet load, tubing and waste-container conditions.
Actual supply and removalMeasure incoming liquid, residue and emptying at the station; check handling after full level, shortage or blockage.

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
DPL30 liquid diaphragm pumpLiquid supply or auxiliary circulationSelect by installed resistance, actual wash or working fluid and task window
DPL60 liquid diaphragm pumpLiquid supply or auxiliary circulationJudge by peak demand and installed operating point, not free flow alone
DPL30H high-backpressure liquid diaphragm pumpLiquid branches requiring higher backpressure capabilityCalculate pressure budget and actual flow first, then confirm interfaces and materials
  • DPL30 liquid diaphragm pump
  • DPL60 liquid diaphragm pump
  • DPL30H high-backpressure liquid diaphragm pump

06Confirm product roles in actual operation

Compare viability, recovery and cell condition before and after transfer, and validate the required contamination controls.

07Do similar nominal supply- and waste-pump flow rates make a matched pair?

Compare peak inflow, effective aspiration and permissible accumulation in the actual installation. The branches may handle different media and operating windows; validate station recovery over the complete sequence.

Low shear and sterility are validation objectives for the complete flow path. Neither a diaphragm pump nor a pinch valve alone establishes them.

References

View references (3)
  1. [1]FOREACH DPL30 liquid diaphragm pump: configurations and specifications
  2. [2]FOREACH DPL60 liquid diaphragm pump: configurations and specifications
  3. [3]FOREACH DPL30H high-backpressure liquid diaphragm pump: configurations and specifications

Related guides

  • Back to cell culture and transfer fluidics

Related Products

DPL30 liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPL30 liquid diaphragm pump

DPL60 liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPL60 liquid diaphragm pump

DPL30H high-backpressure liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPL30H high-backpressure liquid diaphragm pump

Related Technical Articles

How to Read a Diaphragm Pump Flow-Pressure Curve—and Predict Installed Flow

A catalogue value such as 300 or 600 mL/min is not a fixed flow rate inside an instrument. The useful engineering question is: at the actual inlet pressure, outlet backpressure, fluid, tubing and supply voltage, where will the pump and the fluidic circuit settle?

2026-07-28View Details

Selecting a Self-Priming Miniature Liquid Diaphragm Pump: Lift, Priming Time and Dry or Wet Starts

Select a self-priming miniature liquid diaphragm pump by checking suction lift, time to first liquid, dry and wet starting conditions, and stable delivery. Use DPL30, DPL60 and DPL30H specifications to define an instrument-level test.

2026-09-07View Details

What Is a Programmable Syringe Pump? Operation, Fluid Routing and Automation

A programmable syringe pump uses a motor to control syringe plunger displacement and speed, executing aspiration, dispensing, pauses and repeated actions through commands. With a routing valve, it can connect reagent reservoirs, reaction vessels and wash paths into an automated dispensing process. Instrument development therefore needs to define volume, fluid connections and timing together.

2026-09-28View Details

Discuss a cell culture fluidic configuration

Provide a fluid-path diagram, actual liquids and pressures in each state. Select for installed resistance, actual wash or working liquid and the task window. Assess peak demand and installed operating points, not free flow alone. Calculate pressure budgets and actual flow before confirming interfaces and materials, using the validation objectives in this guide.

Contact an engineer