What do these products do in this instrument?
Syringe pumps: periodic replenishment and discrete transfer
Drive aspiration and dispensing for discrete culture-medium replenishment or suitable transfer tasks. Coordinate vessel identity and replenishment timing. For cell-contact paths, separately assess bore size, residence time and transfer outcomes.
HLD and HMD organize aspiration/dispensing paths with rotary and solenoid valves respectively. Capacity budgets include useful volume, holding stages and replenishment points; valve states and pump actions together create each delivery.
Organize syringe metering around aspiration, holding and dispensing
Syringe drives use controlled displacement for aspiration, holding and dispensing. With direct contact, source liquid enters the metering chamber; with system-liquid drive, sample can remain at the probe tip or a designated tube segment. Define wetted coverage before capacity, cleaning and replenishment. For repeated dispensing, track current inventory, delivered volume and next replenishment time.
Map valve connections to every action
Check port connections, opening/closing sequence and power-off state separately for rotary- and solenoid-valve configurations. Changing source does not automatically remove old liquid from the chamber or shared tubing. Plan flushing for formulation and residue requirements, then evaluate the first dose after switching and dose-to-dose variation.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Stored volume and timing | Define volume per operation, consecutive dispensing count, holding time and permitted replenishment window. |
| Valve positions and destinations | Provide a source, receiver and waste port diagram with open/closed and power-off states. |
| Liquid changes and first dose | Check shared residue, priming destination and actual received volume after standby or source changes. |
| Operation | Specific operating conditions |
|---|---|
| Suspension transfer | Cell density, flow rate/pressure differential, bore size, residence time and condition before transfer |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| HLD rotary-valve syringe pump | Aspiration, holding and dispensing with a rotary valve | Check source/outlet valve positions, capacity budget and replenishment timing |
| HMD solenoid-valve syringe pump | Metered aspiration and dispensing with solenoid valves | Check open/closed and power-off states, working volume and replenishment cycle |
Explore specific operating and validation tasks
Suspension transfer
A smaller bore, higher pressure differential or changed local velocity alters the conditions experienced by cells. Compare viability, recovery and aggregation before and after transfer to establish an acceptable operating range.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Suspension transfer | Compare cell viability, recovery and required cell attributes before and after transfer; verify sealing and contamination control through the complete flow path. | Air aspiration, clots/particles, probe contact with a wall or leakage alter actual delivery |
Compare viability, recovery and cell condition before and after transfer, and validate the required contamination controls.
For repeated dispensing from one chamber, is checking total volume enough?
Measure each received dose, especially the first and last. Even when total volume is near target, individual errors may differ. Relate replenishment, valve switching and receiving actions to each result.
Low shear and sterility are validation objectives for the complete flow path. Neither a diaphragm pump nor a pinch valve alone establishes them.