Where path switching occurs in the instrument
Create a state table for lysis, binding, washing, elution and waste. Flush shared segments before switching, protect product channels against backflow and unintended wash entry, and assess contamination against the experiment's purpose.
Use a port-state table to verify product-channel connections during operation, cleaning and shutdown. Define the destination of liquid displaced from shared sections.
Product responsibilities in this operation
Valves control the current source or destination; the associated drive performs metering or transfer. Port connections, actual positioning and shared-segment renewal must match the operation.
For this nucleic acid processing task, confirm all ports, valve states, pressures in each state, shared volume and displacement volume.
Organize the sequence by actual architecture
- For fixed-valve configurations, identify lysis, binding, washing, elution and maintenance sources, and define product-collection and waste outlets separately.
- Check the actual connections in each valve state, coordinating source or destination switching with the drive at allowable pressures.
- Direct old liquid from shared segments to the specified destination and confirm arrival of the new liquid. Before collecting product, check unintended wash entry, backflow and contamination blanks after challenging switching sequences.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | All ports, valve states, pressures in each state, shared volume and flushing volume |
| Acceptance target | Check unintended connections, leakage, backflow and post-switching residue in each state; measure flushing volume and arrival delay through the complete path |
| Fault localization | Misalignment, shared-segment residue or abnormal switching pressure may affect the next task |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| MRV3 multiport rotary valve | Multiple-source selection | Compare 10-, 16- and 24-port options and port diagrams; verify high-pressure suitability separately |
Validate operation results at the receiver
Check unintended connections, leakage, backflow and post-switching residue in each state; measure flushing volume and arrival delay through the complete path.
Record product volume, recovery and downstream results, and check sample-to-sample contamination.
Locate abnormalities and restore the next operation
Misalignment, shared-segment residue or abnormal switching pressure may affect the next task.
Stop the pump, check port states and flush again after recovery.
Extraction and library-preparation liquid handling differ in contamination and outcome requirements. Confirm research workflows and clinical diagnostic methods separately.