What do these products do in this instrument?
Tubing and fittings: complete-path recovery evaluation
Connect sources, pumps, valves and product receivers in a fixed path. Include material surfaces, fittings and added internal tubing volume in low-volume recovery and sample-change checks.
Tubing and fittings together form sealed connections and the actual transfer path. Check formulation, inner/outer diameters, length, tube-end positioning and port geometry, including added volume in residue and arrival delay.
Design tubing and fittings as a complete wetted path
Tubing, fittings and ports jointly determine sealing, transfer volume and medium-contact coverage. Materials and dimensions must suit formulation, operating conditions and installation. Include tubing length and interface volume in priming, shared residue and arrival delay. After connection changes, recheck delivery and cleaning through the actual path.
Calculate connection volume and post-maintenance recovery
Shared volume affects liquid arrival and flushing; interface gaps may retain the previous composition. Record length, actual bore and tube insertion position. After disassembly, reprime and check seals, then compare first-cycle delivery, blanks or recovered product to confirm path recovery.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Materials and assembly | Provide formulation, tubing inner/outer diameters, length, fitting structure and port fit. |
| Volume and load | Calculate shared and replacement-component added volumes; measure actual flow and response. |
| Maintenance recovery | Record disassembly locations and added volume; recheck priming, sealing and first-cycle received results. |
| Operation | Specific operating conditions |
|---|---|
| Connection materials | Tubing ID/OD, length, interface geometry, pressure, materials and maintenance method |
Connection materials
Evaluate adsorption and residual liquid for the nucleic acid, vessels and complete workflow. Select tube bore, material, fitting geometry and replacement procedure, then measure product recovery through the assembled path.
Include tubing, probes, valves and fittings in recovery tests. Before shortening tubing, confirm that installation, maintenance access and receiving geometry remain feasible.
Test sealing and actual delivery, and compare analyte recovery and blanks through the complete flow path.
Adsorption, retention or maintenance contamination can reduce recovery even when volume passes. Isolate the affected section and recheck assembly. Matching threads alone do not establish interchangeability.
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| Tubing materials and dimensions | Connection paths and shared volume | Bore, length, load and actual formulation jointly determine materials and dimensions |
| Rigid-tubing fittings | Tube-end positioning and sealed connections | Check tube outer diameter, threads, seal structure and port-bottom geometry |
Related operations and architecture comparisons
Sample preparation
Assign container identities and destinations to each step, and establish programs for the actual lysis and binding formulations. After an interruption, confirm whether the product has already been transferred before deciding whether to complete the step or stop.
Washing and elution
Adjust aspiration height to the solid-phase position and vessel geometry, and assess low-volume collection separately for elution. Evaluate recovery, residual liquid and the next reaction. Volume accuracy alone does not establish washing effectiveness.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Connection materials | Test sealing and actual delivery, and compare analyte recovery and blanks through the complete flow path. | Adsorption, hold-up or maintenance contamination can reduce recovery despite acceptable volume |
Record product volume, recovery and downstream results, and check sample-to-sample contamination.
Does a fitting that tightens prove the tubing assembly is suitable?
Also confirm port geometry, tube-end positioning and seal fit. Check leakage, hold-up and performance after repeated maintenance under actual conditions; thread engagement alone is insufficient.
Extraction and library-preparation liquid handling differ in contamination and outcome requirements. Confirm research workflows and clinical diagnostic methods separately.