What do these products do in this instrument?
Air-Displacement Pipetting Pumps: Disposable-Tip Transfer of Samples, Reagents and Products
Drive transfers of samples, lysis or binding reagents, and eluates in disposable-tip workflows. Coordinate with well positions, consumables and solid-phase retention steps, and set separate actions for discarding wash liquid and recovering product.
SMTP serves disposable-tip air-displacement architectures, with the pump displacing an air column. Tip sealing, liquid type, immersion position and platform motion jointly determine received volume.
Coordinate air-displacement drive with tips and motion
Air-displacement pipetting pumps move liquid in tips through air-column displacement. Sealing after pickup, tip specification, immersion and platform motion all contribute to aspiration and dispensing. Describe tip-based operations separately from fixed wetted tubing, identifying source well, receiving well and consumable state for each step.
Develop pipetting sequences for actual liquids and receiving actions
Match aspiration speed, waits, air gaps and dispensing mode to the specific liquid and container. Low remaining volume, foam, droplets and post-tip-change state may affect reception. Schedule tip changes according to contamination-control requirements; when discarding liquid or recovering nucleic-acid eluate, verify destination together with sample identity.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Tips and sealing | Provide tip specifications, pickup/ejection method and sealing checks after pickup. |
| Liquid and position | Record liquid type, minimum remaining volume, immersion depth and receiving position. |
| Results by well | Check received volume, empty wells or residual liquid, and tip-change and well records. |
| Operation | Specific operating conditions |
|---|---|
| Sample preparation | Dose per operation, total cycle volume, replenishment/bottle-change interval, backpressure and receiving geometry |
| Washing and elution | Solid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Explore specific operating and validation tasks
Sample preparation
Assign container identities and destinations to each step, and establish programs for the actual lysis and binding formulations. After an interruption, confirm whether the product has already been transferred before deciding whether to complete the step or stop.
Washing and elution
Adjust aspiration height to the solid-phase position and vessel geometry, and assess low-volume collection separately for elution. Evaluate recovery, residual liquid and the next reaction. Volume accuracy alone does not establish washing effectiveness.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Sample preparation | Use actual media to check first, middle and last doses and delivery after bottle changes; confirm received volume and timing rather than substituting commanded steps for volume | Empty sources, bubbles, nozzle droplets and backpressure changes affect first or last doses |
| Washing and elution | Evaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery together | Insufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery |
Record product volume, recovery and downstream results, and check sample-to-sample contamination.
Does completed pump movement confirm that the target well received the specified volume?
Also check tip sealing, air aspiration, well position and dispensing state, and measure actual reception. The drive-complete signal can assess the operation only together with these records.
Extraction and library-preparation liquid handling differ in contamination and outcome requirements. Confirm research workflows and clinical diagnostic methods separately.