Where washing and elution occur in the instrument
Identify the separation architecture first. When pipetting liquid while retaining the solid phase, plan supernatant removal, residual-wash removal, elution and product collection separately. When magnetic rods move beads, organize actions around reagent wells and solid-phase movement. For liquid-driven steps, check aspiration height, the complete wetted path and actual product receipt.
Product responsibilities in this operation
The air-displacement pump drives liquid inside the tip; the platform manages tips, wells and movement. Associate liquid destinations and consumable states with every operation.
For this nucleic acid processing task, define solid-phase position and retention target, wash cycles, aspiration height, residual liquid, destinations and downstream method requirements.
Organize the sequence by actual architecture
- Confirm a tip-based liquid-transfer architecture that retains the solid phase, and mark separate destinations for wash waste and eluate. Organize magnetic-rod bead transfers around the corresponding solid-phase movements.
- Set aspiration height from solid-phase position and vessel geometry. Remove supernatant, add wash liquid and remove residual wash liquid separately, without discarding the solid phase that must be retained.
- Complete elution and the required solid-phase separation according to the method, then use a tip to collect the retained product into the designated vessel.
- Record actual product volume, target recovery, residual wash liquid and contamination blanks. Evaluate the workflow through these results and downstream usability; completion of pipetting alone is insufficient.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Solid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements |
| Acceptance target | Evaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery together |
| Fault localization | Insufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Validate operation results at the receiver
Evaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery together.
Record product volume, recovery and downstream results, and check sample-to-sample contamination.
Locate abnormalities and restore the next operation
Insufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery.
Review actual results of affected steps before resuming the next action.
Extraction and library-preparation liquid handling differ in contamination and outcome requirements. Confirm research workflows and clinical diagnostic methods separately.