Where medium switching occurs in the instrument
Perform medium switching, bottle-change priming and washing as separate steps. Check formulations, shared-path residue and the first delivery after restart. Confirm when the new medium reaches the culture vessel and in what condition.
Bottle changes, priming and switching are different events. Calculate shared volume, then verify arrival time and initial composition to prevent cleaning residue entering the culture.
Product responsibilities in this operation
Valves control the current source or destination; the associated drive performs metering or transfer. Port connections, actual positioning and shared-segment renewal must match the operation.
For continuous feeding, define all ports, valve states, pressures in each state, shared volume and displacement volume.
Organize the sequence by actual architecture
- List medium changeover, bottle-change priming and cleaning states separately. Verify source formulation, culture-side destination and maintenance routing.
- Switch sources in coordination with the drive and actual pinch-off requirements, directing the previous formulation or wash liquid in shared sections to its designated destination.
- Validate arrival time and initial composition at the culture interface. Record interruptions and changes in cumulative delivery during switching.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | All ports, valve states, pressures in each state, shared volume and flushing volume |
| Acceptance target | Check unintended connections, leakage, backflow and post-switching residue in each state; measure flushing volume and arrival delay through the complete path |
| Fault localization | Misalignment, shared-segment residue or abnormal switching pressure may affect the next task |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| MRV3 multiport rotary valve | Multiple-source selection | Compare 10-, 16- and 24-port options and port diagrams; verify high-pressure suitability separately |
Validate operation results at the receiver
Check unintended connections, leakage, backflow and post-switching residue in each state; measure flushing volume and arrival delay through the complete path.
Record output across the run, replenishment interruptions, first flow after bottle changes and reactor volume balance.
Locate abnormalities and restore the next operation
Misalignment, shared-segment residue or abnormal switching pressure may affect the next task.
Stop the pump, check port states and flush again after recovery.
Refilling or resetting a single syringe can interrupt supply. Continuous-operation capability and aseptic isolation both require system validation.