What do these products do in this instrument?
Check valves: restrict backflow in feed branches
Limit flow direction where backflow protection is required, accounting for culture-side backpressure and the pump operating point. Check opening load, reverse leakage and added resistance together.
Check valves limit reverse flow in the permitted direction. Assess opening conditions, reverse leakage and added resistance together with the pump operating point. Check siphoning and the complete path's contamination-control boundary separately.
Tubing and Fittings: Extended-Run Feed Paths and Loads
Connect feed vessels, metering drives and culture interfaces, confirming materials for formulation and prolonged contact. Evaluate tubing and protective-component resistance alongside bottle-change priming and arrival delay.
Tubing and fittings together form sealed connections and the actual transfer path. Check formulation, inner/outer diameters, length, tube-end positioning and port geometry, including added volume in residue and arrival delay.
Design tubing and fittings as a complete wetted path
Tubing, fittings and ports jointly determine sealing, transfer volume and medium-contact coverage. Materials and dimensions must suit formulation, operating conditions and installation. Include tubing length and interface volume in priming, shared residue and arrival delay. After connection changes, recheck delivery and cleaning through the actual path.
Validate check-valve opening load and closed-state sealing separately
Position check valves for the permitted flow direction and include opening differential pressure and added resistance in the actual supply load. Measure normal delivery, reverse leakage after stopping and recovery after maintenance separately. Assess siphoning and the complete path's contamination-control boundary independently.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Materials and assembly | Provide formulation, tubing inner/outer diameters, length, fitting structure and port fit. |
| Volume and load | Calculate shared and replacement-component added volumes; measure actual flow and response. |
| Backflow prevention and load | Check opening differential pressure, actual supply, reverse leakage and sealing after replacement. |
| Operation | Specific operating conditions |
|---|---|
| Flow-path protection | Check-valve cracking pressure, reverse leakage, filter pressure drop, actual load and maintenance state |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| G-series check valves | Backflow limitation | The card shows a representative configuration. Confirm actual opening conditions, reverse leakage and medium separately. |
| Tubing materials and dimensions | Connection paths and shared volume | Bore, length, load and actual formulation jointly determine materials and dimensions |
| Rigid-tubing fittings | Tube-end positioning and sealed connections | Check tube outer diameter, threads, seal structure and port-bottom geometry |
Explore specific operating and validation tasks
Flow-path protection
Check-valve opening pressure, filter pressure drop and backpressure affect the pump operating point. Backflow prevention and sterile isolation require separate validation.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Flow-path protection | Measure opening load, normal flow, reverse leakage and recovery after replacement; validate sterile boundaries separately. | Excessive opening pressure, reverse leakage or filter blockage can disturb feeding. |
Record output across the run, replenishment interruptions, first flow after bottle changes and reactor volume balance.
Does a fitting that tightens prove the tubing assembly is suitable?
Also confirm port geometry, tube-end positioning and seal fit. Check leakage, hold-up and performance after repeated maintenance under actual conditions; thread engagement alone is insufficient.
Refilling or resetting a single syringe can interrupt supply. Continuous-operation capability and aseptic isolation both require system validation.