Where path isolation fits in the instrument
Create a port-state table for feeding, sampling, washing and drainage. Check shared volume, closed-state isolation and unintended connections to prevent wash liquid or waste from returning to the culture vessel.
The port-state table must cover feeding, sampling, cleaning and drainage. Check leakage and backflow even in closed states.
Product responsibilities in this operation
Valves control the current source or destination; the associated drive performs metering or transfer. Port connections, actual positioning and shared-segment renewal must match the operation.
For this microbioreactor task, confirm all ports, valve states, pressures in each state, shared volume and displacement volume.
Organize the sequence by actual architecture
- Draw port and pinch states for each reactor's feeding, sampling, washing and drainage paths. Identify permitted concurrency and independent isolation requirements.
- Coordinate source or destination switching with the drive and confirm tubing-closure position, preventing wash liquid, waste or other formulations entering the culture vessel.
- Check shared-volume renewal, closed-state leakage and backflow. Record affected reactors and completed operations before proceeding to the next stage.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | All ports, valve states, pressures in each state, shared volume and flushing volume |
| Acceptance target | Check unintended connections, leakage, backflow and post-switching residue in each state; measure flushing volume and arrival delay through the complete path |
| Fault localization | Misalignment, shared-segment residue or abnormal switching pressure may affect the next task |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| MRV3 multiport rotary valve | Multiple-source selection | Compare 10-, 16- and 24-port options and port diagrams; verify high-pressure suitability separately |
Validate operation results at the receiver
Check unintended connections, leakage, backflow and post-switching residue in each state; measure flushing volume and arrival delay through the complete path.
Check cumulative feeding, sampling representativeness, volume balance and recovery during long runs.
Locate abnormalities and restore the next operation
Misalignment, shared-segment residue or abnormal switching pressure may affect the next task.
Stop the pump, check port states and flush again after recovery.
Bubble and pressure signals describe fluid-path conditions; they do not directly measure pH, dissolved oxygen or growth. Validate the complete aseptic boundary separately.