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Home/Applications/Synthetic biology/Microbioreactors
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Valveless metering pumps in microbioreactors

Application guide

This page explains valveless metering pump location, coordination and configuration requirements for suitable repeated or continuous feeding in microbioreactors, with links to relevant operating and validation tasks.

Application guides

Microbioreactor application guide

Microbioreactor fluidics overview
Syringe pumps in microbioreactors
Sampling
Valveless metering pumps in microbioreactors
Feeding
Diaphragm pumps in microbioreactors
Air-containing waste aspiration and post-maintenance restart
Fluidic valves in microbioreactors
Path isolation
Bubble and pressure detection in microbioreactors
Tubing and Fittings for Microbioreactors

ON THIS PAGE

What do these products do in this instrument?Distinguish repeated dosing from continuous supply firstHandle starts/stops, replenishment and two-stream merging separatelyTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageExplore specific operating and validation tasksConfirm product roles in actual operationCan the same configuration be retained after changing the target dose or time window?ReferencesRelated guides

01What do these products do in this instrument?

Valveless metering pumps: suitable repeated or continuous feeding

Provide repeated or continuous feed output within actual formulation and backpressure limits, coordinated with feed vessels and process stages. Check time-window flow, cumulative volume and bottle-change recovery separately.

Compare RPL-series options for repeated metering or auxiliary continuous supply according to the task. Confirm individual dose, flow over the operating time window and cumulative volume separately. For proportioning, also compare the liquid actually arriving from each of the two paths.

02Distinguish repeated dosing from continuous supply first

Compare valveless metering pumps for repeated dosing or auxiliary continuous supply in suitable configurations. Repeated dosing requires assessment of individual deliveries and liquid released after stopping; continuous tasks require time-window flow and cumulative volume. After converting the rotation program to a set volume, measure the actual received volume under the installed flow-path and load conditions.

03Handle starts/stops, replenishment and two-stream merging separately

Starting, stopping and bottle changes affect actual arrival. Record initial liquid delivery, recovery delay and terminal drips. For two-stream proportioning, check each path's load and arrival time before checking the composition and mixing after the junction. A matching cumulative ratio does not replace composition validation during startup and within each operating time window.

04Translate actual branch conditions into configuration requirements

Valveless metering-pump configuration for microbioreactors
ConditionTo confirm
Output requirementsDefine individual dose or target flow, duration, cumulative volume and allowable interruptions.
Installed loadSpecify medium, inlet conditions, outlet backpressure and downstream connections.
Start-stop behavior and proportioningMeasure initial, steady-state and restart output. For two-stream tasks, also check actual arrival and mixing results.
Operations supported in this instrument
OperationSpecific operating conditions
FeedingDiscrete or continuous feeding mode, time profile, medium, load, cumulative volume and permissible interruption

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
RPL Valveless Metering PumpSuitable repeated or continuous meteringCompare displacement, speed, actual backpressure and starts/stops; check two-stream ratios separately
  • RPL Valveless Metering Pump

06Explore specific operating and validation tasks

Feeding

Adding the same cumulative volume at different times may alter the process. Translate batch, periodic-pulse or continuous requirements into allowable variation and interruptions before comparing drives.

  • Feeding

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
FeedingMeasure pulse doses or flow over defined time windows separately, then reconcile cumulative material, bottle changes and restarts.An empty source, bubbles, refill interruptions or repeated control states may alter actual feed delivery.

Check cumulative feeding, sampling representativeness, volume balance and recovery during long runs.

08Can the same configuration be retained after changing the target dose or time window?

Validate starts/stops, operating increments and receiver-end drips under the new actual load. Measure repeated dispensing dose by dose; for continuous supply, measure output within operating time windows and cumulatively. Then determine whether the existing configuration and program still meet requirements.

Bubble and pressure signals describe fluid-path conditions; they do not directly measure pH, dissolved oxygen or growth. Validate the complete aseptic boundary separately.

References

View references (1)
  1. [1]FOREACH RPL valveless metering pumps: configurations and specifications

Related guides

  • Back to microbioreactor fluidics
  • Feeding

Related Products

RPL Valveless Metering Pump; representative configuration. Select capacity, materials and interfaces for the application.

RPL Valveless Metering Pump

Related Technical Articles

What Is a Valveless Metering Pump? Working Principle, Applications and Selection

A valveless metering pump is a positive-displacement pump that combines rotary and axial reciprocating piston motion to switch the fluid path, draw liquid in and discharge it. Its pumping mechanism does not rely on conventional inlet and outlet check valves. It can serve reagent dispensing, titration, filling and two-fluid proportional delivery, but metering performance still has to be verified with the actual fluid, pressure, tubing, drive and cleaning conditions.

2026-09-21View Details

Valveless Metering Pump Selection Guide: FOREACH Models and Applications

To select a valveless metering pump, first define the volume per dose and the time available for dispensing. Then check displacement per revolution, drive speed, wetted materials, backpressure and connections, and verify delivery in the actual fluid path. Neither flow rate alone nor the label ‘metering pump’ is enough to select a model.

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What Is a Programmable Syringe Pump? Operation, Fluid Routing and Automation

A programmable syringe pump uses a motor to control syringe plunger displacement and speed, executing aspiration, dispensing, pauses and repeated actions through commands. With a routing valve, it can connect reagent reservoirs, reaction vessels and wash paths into an automated dispensing process. Instrument development therefore needs to define volume, fluid connections and timing together.

2026-09-28View Details

Discuss a microbioreactor fluidic configuration

Provide a fluidic diagram, actual liquids and pressures in each operating state. Compare displacement, speed, actual backpressure and start-stop behavior; verify dual-stream ratios separately. Assess suitable configurations against this guide's validation objectives.

Contact an engineer