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Home/Applications/Synthetic biology/Microbioreactors
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Diaphragm pumps in microbioreactors

Application guide

This page explains the diaphragm pump's position, supporting components and configuration requirements for suitable gas-containing waste aspiration in microbioreactors, with links to operating and validation tasks.

Application guides

Microbioreactor application guide

Microbioreactor fluidics overview
Syringe pumps in microbioreactors
Sampling
Valveless metering pumps in microbioreactors
Feeding
Diaphragm pumps in microbioreactors
Air-containing waste aspiration and post-maintenance restart
Fluidic valves in microbioreactors
Path isolation
Bubble and pressure detection in microbioreactors
Tubing and Fittings for Microbioreactors

ON THIS PAGE

What do these products do in this instrument?First identify the actual connection architecture for gas-containing aspirationCombine suction/drainage and vessel protection into a complete cycleTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageExplore specific operating and validation tasksConfirm product roles in actual operationCan an air-containing aspiration pump be selected from liquid-flow requirements alone?ReferencesRelated guides

01What do these products do in this instrument?

Gas-Liquid Diaphragm Pumps: Suitable Gas-Containing Waste Aspiration

Serve waste removal in architectures with actual air-containing inlet flow or vessel vacuum, accounting for biomass, foam and peak inflow. Confirm medium suitability for the specific configuration, and check sealing and isolation after maintenance.

DPGL800 handles gas or gas–liquid aspiration permitted by its specific configuration. Check inlet gas content, foam, peak inflow and container vacuum; use a separately suitable solution for continuous liquid-only transfer.

02First identify the actual connection architecture for gas-containing aspiration

During direct aspiration, the permitted gas-liquid mixture passes through the pump. In vacuum-over-liquid collection, liquid enters a waste vessel and the pump connects to its gas space. Confirm inlet media, biomass or foam, vacuum and discharge destination for the configuration.

03Combine suction/drainage and vessel protection into a complete cycle

Check actual drainage against peak inflow, allowable accumulation and the available aspiration window. Test interlocks for full vessels, foam, leaks and blockage. After maintenance, verify sealing and isolation before continuing. Select separate suitable configurations for continuous liquid-only transfer and wash supply.

04Translate actual branch conditions into configuration requirements

Diaphragm-pump configuration requirements for microbioreactors
ConditionTo confirm
Inlet mediumDistinguish liquid-only flow, intermittent gas entrainment and foam, and confirm the aspiration modes permitted by the configuration.
Tubing and containersProvide suction lift, outlet load, tubing and waste-container conditions.
Actual supply and removalMeasure incoming liquid, residue and emptying at the station; check handling after full level, shortage or blockage.
Operations supported in this instrument
OperationSpecific operating conditions
Air-containing waste aspiration and post-maintenance restartLiquid or gas-liquid inlet conditions, peak incoming flow, allowable accumulation, lift and waste container

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
DPGL800 gas–liquid diaphragm pumpSuitable gas–liquid aspiration or container-vacuum branchesConfirm inlet medium and waste architecture; not for continuous liquid-only transfer (100% liquid)
  • DPGL800 gas–liquid diaphragm pump

06Explore specific operating and validation tasks

Air-containing waste aspiration and post-maintenance restart

Confirm suitable conditions separately for air-containing aspiration and liquid-only drainage. After maintenance, check sealing and isolation before resuming culture operations.

  • Air-containing waste aspiration and post-maintenance restart

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Air-containing waste aspiration and post-maintenance restartTest emptying, liquid accumulation and interlocks after a full-vessel or blockage condition using the actual incoming liquid.Leaks, foam, blockages or a full container can reduce actual drainage capacity.

Check cumulative feeding, sampling representativeness, volume balance and recovery during long runs.

08Can an air-containing aspiration pump be selected from liquid-flow requirements alone?

Check the specific configuration against actual inlet phase, vacuum and operating period, then measure installed drainage and container conditions. Gas or gas-liquid aspiration specifications do not establish continuous liquid-only transfer capability.

Bubble and pressure signals describe fluid-path conditions; they do not directly measure pH, dissolved oxygen or growth. Validate the complete aseptic boundary separately.

References

View references (1)
  1. [1]FOREACH DPGL800 gas–liquid diaphragm pump: configurations and specifications

Related guides

  • Back to microbioreactor fluidics
  • Air-containing waste aspiration and post-maintenance restart

Related Products

DPGL800 gas–liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPGL800 gas–liquid diaphragm pump

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Discuss a microbioreactor fluidic configuration

Provide a fluidic diagram, actual liquids and pressures in each operating state. Identify the inlet medium and waste-removal architecture; the gas-liquid option is not for continuous transfer of 100% liquid. Compare configurations against this guide's validation objectives.

Contact an engineer