Where tip-based sample transfer occurs in the instrument
This task covers disposable-tip air displacement on sample preparation platforms. The pipetting pump drives liquid in the tip while the platform coordinates sources, destinations and consumables. Verify the pickup seal, actual liquid and sample identity before setting aspiration, dispensing and motion programs.
Start liquid actions only after the probe or tip reaches position. After aspiration or dispensing, check retained droplets and receiving status. Recheck these conditions when increasing workstation speed.
Product responsibilities in this operation
The air-displacement pump drives liquid inside the tip; the platform manages tips, wells and movement. Associate liquid destinations and consumable states with every operation.
For tip-based sample transfer, confirm disposable-tip specification and pickup sealing, actual liquid class, source volume remaining, immersion depth, working volume and receiving position.
Organize the sequence by actual architecture
- For this disposable-tip workstation task, verify source sample, destination vessel, tip specification and sealing after pickup. Configure fixed-probe architectures separately around their wetted paths and washing procedures.
- Set immersion depth, aspiration speed, dwell time and air gaps for the actual liquid class. Check source volume and destination before aspirating.
- Move the tip to the designated vessel with the platform, dispense according to the receiving procedure, and record actual volume, retained droplets or air aspiration with the corresponding sample identity.
- Change and eject tips according to contamination controls, then transfer delivery and sample-state records to the separation or reaction station. After interruption, check for partial delivery first.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Disposable-tip specification and pickup seal, actual liquid class, source volume remaining, immersion depth, working volume and receiving position |
| Acceptance target | Measure actual receipt at the target vessel, including low source levels, tip pickup and ejection, and contamination across sample sequences. Verify sample or well identity and downstream method results. |
| Fault localization | Tip leaks, aspiration of air, hanging droplets, incorrect immersion or missed tip changes can affect delivery and contamination. |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Validate operation results at the receiver
Measure actual volume received in the target vessel, covering low remaining volume, tip pickup/disposal and contamination across sample sequences. Verify sample or well identity and subsequent method results.
Validate working volume, timing across workstations, sample identity and the first operation after restart together.
Locate abnormalities and restore the next operation
Tip air leaks, air aspiration, retained droplets, incorrect immersion or missed tip changes can affect delivery and contamination status.
Check the volume already delivered and the destination vessel first. Handle the tip and affected sample according to the experimental procedure, then verify readiness before the next action.
Successful platform operation does not replace assessment of nucleic acid, cell or analytical method results.