Where washing and product recovery occur in the instrument
Coordinate wash supply, aspiration, elution and waste resources across stations. Maintain defined product and waste destinations, assess residual liquid and recovery against method results, and include priming and washing in cycle timing.
When several stations wash simultaneously, shared supply and waste removal can become bottlenecks. Calculate peak demand first, then assess residual liquid and recovery through the complete workflow.
Product responsibilities in this operation
Diaphragm pumps perform suitable liquid supply or aerated aspiration in this task. Confirm inlet phase, branch location and outlet destination separately, then check each branch's operating point.
Compare DPL30, DPL60 and DPL30H by actual supply load. Wash-head, valve, tubing and container resistance jointly determine installed flow; check the supply window and receiving position together.
DPGL800 handles gas or gas–liquid aspiration permitted by its specific configuration. Check inlet gas content, foam, peak inflow and container vacuum; use a separately suitable solution for continuous liquid-only transfer.
For washing and product recovery, confirm solid-phase location and retention target, wash cycles, aspiration height, residual liquid, destinations and downstream requirements.
Organize the sequence by actual architecture
- Identify the solid phase or product at each station, wash-liquid sources, waste destinations and eluate receivers. Confirm the separation architecture used.
- Reserve the pipetting, supply or drainage resources required by the workstation configuration. Confirm reservation and state of shared pumps, valves and wash stations first.
- Perform wash-waste removal, elution and product collection separately according to the method, checking aspiration height and destinations. Organize magnetic-rod transfers around solid-phase movement.
- Check residual liquid, recovery and contamination before releasing shared resources and handing over sample status. On restart, use the completed volume to determine where to resume.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Solid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements |
| Acceptance target | Test the intended concurrent station load on shared wash and drainage resources. At each station, verify solid-phase or product retention, residual liquid and the separate destinations for discarded wash fluid and collected eluate. |
| Fault localization | A shared supply or waste bottleneck can leave one station incompletely washed while another finishes. Incorrect aspiration height or an uncertain destination can lose product despite an apparently completed platform schedule. |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| DPL30 liquid diaphragm pump | Liquid supply or auxiliary circulation | Select by installed resistance, actual wash or working fluid and task window |
| DPL60 liquid diaphragm pump | Liquid supply or auxiliary circulation | Judge by peak demand and installed operating point, not free flow alone |
| DPL30H high-backpressure liquid diaphragm pump | Liquid branches requiring higher backpressure capability | Calculate pressure budget and actual flow first, then confirm interfaces and materials |
| DPGL800 gas–liquid diaphragm pump | Suitable gas–liquid aspiration or container-vacuum branches | Confirm inlet medium and waste architecture; not for continuous liquid-only transfer (100% liquid) |
Validate operation results at the receiver
Test the intended concurrent station load on shared wash and drainage resources. At each station, verify solid-phase or product retention, residual liquid and the separate destinations for discarded wash fluid and collected eluate.
Check method-relevant recovery and contamination results before handing the sample to the next station. Keep resource availability, completed volume and sample status consistent at the handoff.
Locate abnormalities and restore the next operation
A shared supply or waste bottleneck can leave one station incompletely washed while another finishes. Incorrect aspiration height or an uncertain destination can lose product despite an apparently completed platform schedule.
Hold the affected stations and retain their resource reservations and sample identities until states are reconciled. Establish which wash, aspiration or collection actions completed, restore supply and drainage, and resume only the step permitted by the sample state and method.
Successful platform operation does not replace assessment of nucleic acid, cell or analytical method results.
References
View references (4)
- [1]FOREACH DPL30 liquid diaphragm pump: configurations and specifications
- [2]FOREACH DPL60 liquid diaphragm pump: configurations and specifications
- [3]FOREACH DPL30H high-backpressure liquid diaphragm pump: configurations and specifications
- [4]FOREACH DPGL800 gas–liquid diaphragm pump: configurations and specifications