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Home/Applications/Laboratory automation/Sample preparation platforms
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Diaphragm pumps in sample preparation platforms

Application guide

This page explains diaphragm-pump location, coordination and configuration requirements for wash-station/shared supply and air-containing drainage from shared wash stations in sample preparation platforms, with links to relevant operating and validation tasks.

Application guides

Sample preparation platform application guide

Sample preparation platform fluidics overview
Syringe pumps in sample preparation platforms
Air-displacement pipetting pumps in sample preparation platforms
Disposable-tip sample transfer
Diaphragm pumps in sample preparation platforms
Washing and product recovery
Multiport rotary valves in sample preparation platforms
Path scheduling
Bubble and pressure detection in sample preparation platforms
Tubing and fittings in sample preparation platforms

ON THIS PAGE

What do these products do in this instrument?Define liquid-supply and aerated-aspiration branches separatelyMatch supply/removal capacity to washing-station and container conditionsTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageExplore specific operating and validation tasksConfirm product roles in actual operationDo similar nominal supply- and waste-pump flow rates make a matched pair?ReferencesRelated guides

01What do these products do in this instrument?

Liquid Diaphragm Pumps: Wash-Station and Shared Liquid Supply

Supply liquid to shared wash stations or supply vessels present in the actual configuration, coordinated with station occupancy and wash heads. Check delivered supply volume and available windows for individual and concurrent station operation separately.

Compare DPL30, DPL60 and DPL30H by actual supply load. Wash-head, valve, tubing and container resistance jointly determine installed flow; check the supply window and receiving position together.

Gas-liquid diaphragm pumps: air-containing waste aspiration at shared wash stations

Remove incoming wash liquid through suitable gas-containing aspiration or vessel-vacuum paths, allowing for peak waste from each station. Include full containers, accumulated liquid and interruptions in platform interlocks.

DPGL800 handles gas or gas–liquid aspiration permitted by its specific configuration. Check inlet gas content, foam, peak inflow and container vacuum; use a separately suitable solution for continuous liquid-only transfer.

02Define liquid-supply and aerated-aspiration branches separately

Suitable liquid diaphragm-pump configurations supply wash heads, stations or working vessels. Gas–liquid diaphragm pumps serve permitted aerated aspiration or related container-vacuum architectures. Describe actual inlet medium and gas–liquid state first, then determine pump position, incoming source and discharge destination.

03Match supply/removal capacity to washing-station and container conditions

Installed flow depends jointly on pump, tubing, valves, wash head and container; free flow does not directly represent station delivery. Evaluate supply by effective coverage and available time, and waste removal by peak inflow, lift, permitted residual liquid and full-container state. Check the complete wash cycle when supply and aspiration must be coordinated.

04Translate actual branch conditions into configuration requirements

Diaphragm-pump configuration for sample preparation platforms
ConditionTo confirm
Inlet mediumDistinguish liquid-only flow, intermittent gas entrainment and foam, and confirm the aspiration modes permitted by the configuration.
Tubing and containersProvide suction lift, outlet load, tubing and waste-container conditions.
Actual supply and removalMeasure incoming liquid, residue and emptying at the station; check handling after full level, shortage or blockage.
Operations supported in this instrument
OperationSpecific operating conditions
Washing and product recoverySolid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
DPL30 liquid diaphragm pumpLiquid supply or auxiliary circulationSelect by installed resistance, actual wash or working fluid and task window
DPL60 liquid diaphragm pumpLiquid supply or auxiliary circulationJudge by peak demand and installed operating point, not free flow alone
DPL30H high-backpressure liquid diaphragm pumpLiquid branches requiring higher backpressure capabilityCalculate pressure budget and actual flow first, then confirm interfaces and materials
DPGL800 gas–liquid diaphragm pumpSuitable gas–liquid aspiration or container-vacuum branchesConfirm inlet medium and waste architecture; not for continuous liquid-only transfer (100% liquid)
  • DPL30 liquid diaphragm pump
  • DPL60 liquid diaphragm pump
  • DPL30H high-backpressure liquid diaphragm pump
  • DPGL800 gas–liquid diaphragm pump

06Explore specific operating and validation tasks

Washing and product recovery

When several stations wash simultaneously, shared supply and waste removal can become bottlenecks. Calculate peak demand first, then assess residual liquid and recovery through the complete workflow.

  • Washing and product recovery

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Washing and product recoveryEvaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery togetherInsufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery

Validate working volume, timing across workstations, sample identity and the first operation after restart together.

08Do similar nominal supply- and waste-pump flow rates make a matched pair?

Compare peak inflow, effective aspiration and permissible accumulation in the actual installation. The branches may handle different media and operating windows; validate station recovery over the complete sequence.

Successful platform operation does not replace assessment of nucleic acid, cell or analytical method results.

References

View references (4)
  1. [1]FOREACH DPL30 liquid diaphragm pump: configurations and specifications
  2. [2]FOREACH DPL60 liquid diaphragm pump: configurations and specifications
  3. [3]FOREACH DPL30H high-backpressure liquid diaphragm pump: configurations and specifications
  4. [4]FOREACH DPGL800 gas–liquid diaphragm pump: configurations and specifications

Related guides

  • Return to the sample preparation workstation overview
  • Washing and product recovery

Related Products

DPL30 liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPL30 liquid diaphragm pump

DPL60 liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPL60 liquid diaphragm pump

DPL30H high-backpressure liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPL30H high-backpressure liquid diaphragm pump

DPGL800 gas–liquid diaphragm pump; representative configuration. Select capacity, materials and interfaces for the application.

DPGL800 gas–liquid diaphragm pump

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Selecting a Self-Priming Miniature Liquid Diaphragm Pump: Lift, Priming Time and Dry or Wet Starts

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What is a programmable automatic air-displacement pipetting pump?

A programmable automatic air-displacement pipetting pump is a liquid-handling component whose aspiration, dispense, speeds and sequence are set through control commands. This article focuses on air-displacement modules that use disposable tips in OEM instruments, with FOREACH SMTP2 as an example of operation, monitoring and selection limits.

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Discuss fluidic configurations for your sample preparation workstation

Provide a fluidic diagram, actual liquids and pressures in each operating state. Select using installed resistance, actual wash or working liquid and task duration. Assess peak demand and the installed operating point rather than free flow alone. Calculate the pressure budget and actual flow before confirming interfaces and materials. Identify the inlet medium and waste-removal architecture; the gas-liquid option is not for continuous transfer of 100% liquid. Compare suitable configurations against this guide's validation objectives.

Contact an engineer