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Home/Applications/Laboratory automation/Sample preparation platforms
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Bubble and pressure detection in sample preparation platforms

Application guide

Explore bubble- and pressure-sensing locations, coordination and configuration for monitoring supply and transfer anomalies in sample preparation platforms, with links to operating and validation tasks.

Application guides

Sample preparation platform application guide

Sample preparation platform fluidics overview
Syringe pumps in sample preparation platforms
Air-displacement pipetting pumps in sample preparation platforms
Disposable-tip sample transfer
Diaphragm pumps in sample preparation platforms
Washing and product recovery
Multiport rotary valves in sample preparation platforms
Path scheduling
Bubble and pressure detection in sample preparation platforms
Tubing and fittings in sample preparation platforms

ON THIS PAGE

What do these products do in this instrument?Match sensing locations to specific fluidic problemsConnect signals, action windows and recovery conditionsTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageRelated operations and architecture comparisonsConfirm product roles in actual operationCan dispensing be repeated immediately after bubbles or abnormal pressure are detected?ReferencesRelated guides

01What do these products do in this instrument?

Bubble and pressure detection: identify supply and transfer anomalies

Collect pressure or bubble signals in the actual branch. The controller associates them with the current source, workstation and delivery stage. Use different restart rules for partial and completed transfers.

ABD provides bubble or liquid-state signals for defined tubing; PDM5 provides pressure information at a defined location. Configure module, position and action window separately; the controller associates affected samples or tasks.

02Match sensing locations to specific fluidic problems

Bubble modules report bubble or liquid state in defined tube segments; pressure modules report pressure at defined positions. Before selecting mounting points, define the phase and abnormality to observe: aspiration, delivery, source depletion or branch-load changes. Associate sensor positions with affected samples, cuvettes or stations.

03Connect signals, action windows and recovery conditions

The same signal may mean different things at different valve positions or operating phases. Record normal ranges and faults with actual liquids, tubing and sequences. The controller combines action timing, confirmed delivery status, volume records and sample identity to pause, review or recover; one pressure change cannot directly identify a blockage location or specific cause. Distinguish measured, estimated and unknown volumes; completion of a command does not prove actual liquid delivery.

04Translate actual branch conditions into configuration requirements

Bubble and pressure detection configuration requirements for sample preparation platforms
ConditionTo confirm
Installation conditionsSpecify tubing dimensions, liquid, sensing location and branch to observe.
Timing and baselineRecord valve positions, pump actions, normal changes and fault-detection windows.
Abnormality handlingAssociate affected containers with confirmed delivery status and volume records; define conditions for review, continuation or re-execution. Distinguish measured, estimated and unknown volumes; completion of a command does not prove actual liquid delivery.
Operations supported in this instrument
OperationSpecific operating conditions
Washing and product recoverySolid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
ABD bubble-detection moduleBubble or liquid-state identification in defined tubingValidate detection and false alarms for tubing material, dimensions, medium and action window
PDM5 pressure-detection modulePressure-state monitoring at a defined fluidic locationConfirm range and wetted configuration by model; validate normal baseline and abnormalities separately
  • ABD bubble-detection module
  • PDM5 pressure-detection module

06Related operations and architecture comparisons

Washing and product recovery

When several stations wash simultaneously, shared supply and waste removal can become bottlenecks. Calculate peak demand first, then assess residual liquid and recovery through the complete workflow.

  • Washing and product recovery

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Washing and product recoveryEvaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery togetherInsufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery

Validate working volume, timing across workstations, sample identity and the first operation after restart together.

08Can dispensing be repeated immediately after bubbles or abnormal pressure are detected?

First establish whether partial delivery has occurred and which container is affected. Immediate repetition may double-dose it. Act on the actual state and recheck the first cycle after recovery.

Successful platform operation does not replace assessment of nucleic acid, cell or analytical method results.

References

View references (2)
  1. [1]FOREACH ABD bubble-detection module: configurations and specifications
  2. [2]FOREACH PDM5 pressure-detection module: configurations and specifications

Related guides

  • Return to the sample preparation workstation overview

Related Products

ABD bubble-detection module; representative configuration. Select capacity, materials and interfaces for the application.

ABD bubble-detection module

PDM5 pressure-detection module; representative configuration. Select capacity, materials and interfaces for the application.

PDM5 pressure-detection module

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Discuss fluidic configurations for your sample preparation workstation

Provide a fluid-path diagram, actual liquids and pressures in each state. Validate detection and false alarms for the tubing, dimensions, media and action window. Confirm range and wetted configuration by model, then test normal baselines and faults separately against the guide's validation objectives.

Contact an engineer