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Home/Applications/Laboratory automation/Microplate dispensing and washing
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Wash probes in microplate dispensing and washing

Application guide

Explore wash-probe placement, coordination and configuration for well interfaces and aspiration height in microplate dispensing and washing, with links to operating and validation tasks.

Applicable architecture

Solid-phase retention and washing conditions apply to microplate methods that include those tasks. Configure simple dispensing or other plate operations for the actual station. Valve port count is not the number of channels that can independently supply liquid simultaneously.

Application guides

Microplate dispensing and washing application guide

Fluidic Overview for Microplate Dispensing and Washing
Syringe pumps in microplate dispensing and washing
Well dispensing
Diaphragm pumps in microplate dispensing and washing
Microplate washing and aspiration
Solenoid Valves for Microplate Dispensing and Washing
Wash probes in microplate dispensing and washing
Bubble and Pressure Monitoring for Microplate Dispensing and Washing
Tubing and fittings in microplate dispensing and washing

ON THIS PAGE

What do these products do in this instrument?Match component geometry to actual containersEvaluate interfaces by receiving or cleaning resultsTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageRelated operations and architecture comparisonsConfirm product roles in actual operationCan sampling and wash probes be replaced based on similar appearance alone?ReferencesRelated guides

01What do these products do in this instrument?

Wash needles: well-washing interfaces and aspiration height

Provide supply or aspiration interfaces at actual wash stations, coordinated with plate type and motion position. For methods requiring solid-phase retention, also check aspiration height, residual liquid and method background.

Custom wash probes form the container interface for wash-fluid entry or residual-liquid extraction. Confirm supply coverage, aspiration height and drainage timing together with actual container shape.

02Match component geometry to actual containers

The wash needle must suit the vessel opening, bottom, minimum liquid level and motion travel. Record actual contact surfaces and permitted positions before defining the tip, connection and motion clearance; appearance alone is insufficient for selection.

03Evaluate interfaces by receiving or cleaning results

At the source, check reachable positions and low remaining volume; at the receiver, check actual volume, droplets or permitted residue. Test wetted-surface maintenance and recovery for the next container during consecutive operation. Reconfirm position and delivery after changing probes, tubing length or containers.

04Translate actual branch conditions into configuration requirements

Wash-needle configuration requirements for microplate dispensing and washing
ConditionTo confirm
Container and positionProvide opening, bottom, minimum level, positioning error and available motion space.
Liquid contact and surfacesSpecify sample or reagent, residue requirements and internal/external probe cleaning.
Actual functionCheck delivery, coverage, residue, solid-phase retention or mixing results according to the task.
Operations supported in this instrument
OperationSpecific operating conditions
Microplate washing and aspirationSolid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
Custom wash probeWashing interface for probe interiors/exteriors or containersConfirm contaminated surfaces, coverage, aspiration height and peak waste removal
  • Custom wash probe

06Related operations and architecture comparisons

Microplate washing and aspiration

Observe residual liquid, foam and solid-phase loss during each cycle. Insufficient supply, a misaligned wash head and delayed waste removal can cause similar symptoms and must be checked separately.

  • Microplate washing and aspiration

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Microplate washing and aspirationEvaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery togetherInsufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery

Assess differences in delivered volume between wells, residual liquid after washing and the complete plate cycle. Preserve well identities.

08Can sampling and wash probes be replaced based on similar appearance alone?

Confirm tip, channels, interfaces and operating positions separately, then validate each probe's aspiration/dispensing or cleaning results. Similar appearance does not imply equal wetted volume, coverage or residual-liquid levels.

Multiport selection does not provide independent simultaneous channels. Validate washing through both liquid handling and retention of the required solid phase.

References

View references (1)
  1. [1]FOREACH custom wash probes: configurations and specifications

Related guides

  • Back to the microplate dispensing and washing fluidics overview

Related Products

Custom wash probe; representative configuration. Select capacity, materials and interfaces for the application.

Custom wash probe

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Provide a fluid-path diagram, actual liquids and pressures in each state. Identify contaminated surfaces, wash coverage, aspiration height and peak waste demand. Compare configurations against the validation objectives in this guide.

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