Where well dispensing occurs in the instrument
Plan sequence, independent channels and receiving heights for the actual plate format and well layout. Validate delivered volume per well, first/last wells and concurrent-supply differences. Valve port count does not establish simultaneous supply capacity.
Include first, last and edge wells and concurrent operations. Record branch lengths and receiving heights. The port count of a multiport selector valve does not equal the number of simultaneous supply channels.
Product responsibilities in this operation
Syringe drive and valve positions jointly organize aspiration, holding and dispensing. Record working volume, remaining inventory and replenishment timing according to the sequence.
For microplate dispensing, confirm plate format, well positions, independent channels, branch resistance and concurrent flow.
Organize the sequence by actual architecture
- Confirm plate format, target wells, dose per well and cycle time. Identify whether syringe-driven dispensing uses sequential supply or physically independent parallel branches.
- Prepare usable liquid reserves and refill windows for the cycle volume. Check valve position, manifold and dispensing-end position. Port count does not replace the count of independently available simultaneous channels.
- Deliver to the designated wells and check received volume and receiving height individually, including first, last and edge wells and permitted concurrent actions.
- Compare branch resistance, blocked outlets and leaks, then check the first well after replenishment and the complete plate. Preserve well identity so recovery does not cause an entire plate to be dosed again.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Plate format, well positions, independent channels, branch resistance and concurrent flow |
| Acceptance target | Measure first and last wells, channel differences and the complete plate cycle; preserve well identities and verify actual delivery and residual liquid. |
| Fault localization | Empty sources, bubbles, nozzle droplets and backpressure changes affect first or last doses |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| HLD rotary-valve syringe pump | Aspiration, holding and dispensing with a rotary valve | Check source/outlet valve positions, capacity budget and replenishment timing |
| HMD solenoid-valve syringe pump | Metered aspiration and dispensing with solenoid valves | Check open/closed and power-off states, working volume and replenishment cycle |
Validate operation results at the receiver
Test first and last wells, channel variation and the complete plate cycle. Retain well identities and verify actual delivery and residual liquid.
Assess differences in delivered volume between wells, residual liquid after washing and the complete plate cycle. Preserve well identities.
Locate abnormalities and restore the next operation
Empty sources, bubbles, nozzle droplets and backpressure changes may affect first or last doses.
Stop affected steps, validate recovery after priming and avoid duplicate dispensing.
Multiport selection does not provide independent simultaneous channels. Validate washing through both liquid handling and retention of the required solid phase.