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Home/Applications/Laboratory automation/Microplate dispensing and washing
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Bubble and Pressure Monitoring for Microplate Dispensing and Washing

Application guide

Explore sensing locations, coordination and configuration for investigating low-liquid, supply and drainage anomalies in microplate workstations, with links to operating and validation tasks.

Applicable architecture

Solid-phase retention and washing conditions apply to microplate methods that include those tasks. Configure simple dispensing or other plate operations for the actual station. Valve port count is not the number of channels that can independently supply liquid simultaneously.

Application guides

Microplate dispensing and washing application guide

Fluidic Overview for Microplate Dispensing and Washing
Syringe pumps in microplate dispensing and washing
Well dispensing
Diaphragm pumps in microplate dispensing and washing
Microplate washing and aspiration
Solenoid Valves for Microplate Dispensing and Washing
Wash probes in microplate dispensing and washing
Bubble and Pressure Monitoring for Microplate Dispensing and Washing
Tubing and fittings in microplate dispensing and washing

ON THIS PAGE

What do these products do in this instrument?Match sensing locations to specific fluidic problemsConnect signals, action windows and recovery conditionsTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageRelated operations and architecture comparisonsConfirm product roles in actual operationCan dispensing be repeated immediately after bubbles or abnormal pressure are detected?ReferencesRelated guides

01What do these products do in this instrument?

Bubble and pressure sensing: monitoring liquid supply and drainage

Provide pressure and bubble signals in shared or specified branches. The platform can associate signal timing with potentially affected wells and the current task. Normal total supply does not rule out blocked ports, leaks or local resistance.

ABD provides bubble or liquid-state signals for defined tubing; PDM5 provides pressure information at a defined location. Configure module, position and action window separately; the controller associates affected samples or tasks.

02Match sensing locations to specific fluidic problems

Bubble modules report bubble or liquid state in defined tube segments; pressure modules report pressure at defined positions. Before selecting mounting points, define the phase and abnormality to observe: aspiration, delivery, source depletion or branch-load changes. Associate sensor positions with affected samples, cuvettes or stations.

03Connect signals, action windows and recovery conditions

The same signal may mean different things at different valve positions or operating phases. Record normal ranges and faults with actual liquids, tubing and sequences. The controller combines action timing, confirmed delivery status, volume records and sample identity to pause, review or recover; one pressure change cannot directly identify a blockage location or specific cause. Distinguish measured, estimated and unknown volumes; completion of a command does not prove actual liquid delivery.

04Translate actual branch conditions into configuration requirements

Bubble and Pressure Monitoring Configuration for Microplate Dispensing and Washing
ConditionTo confirm
Installation conditionsSpecify tubing dimensions, liquid, sensing location and branch to observe.
Timing and baselineRecord valve positions, pump actions, normal changes and fault-detection windows.
Abnormality handlingAssociate affected containers with confirmed delivery status and volume records; define conditions for review, continuation or re-execution. Distinguish measured, estimated and unknown volumes; completion of a command does not prove actual liquid delivery.
Operations supported in this instrument
OperationSpecific operating conditions
Microplate washing and aspirationSolid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
ABD bubble-detection moduleBubble or liquid-state identification in defined tubingValidate detection and false alarms for tubing material, dimensions, medium and action window
PDM5 pressure-detection modulePressure-state monitoring at a defined fluidic locationConfirm range and wetted configuration by model; validate normal baseline and abnormalities separately
  • ABD bubble-detection module
  • PDM5 pressure-detection module

06Related operations and architecture comparisons

Microplate washing and aspiration

Observe residual liquid, foam and solid-phase loss during each cycle. Insufficient supply, a misaligned wash head and delayed waste removal can cause similar symptoms and must be checked separately.

  • Microplate washing and aspiration

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Microplate washing and aspirationEvaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery togetherInsufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery

Assess differences in delivered volume between wells, residual liquid after washing and the complete plate cycle. Preserve well identities.

08Can dispensing be repeated immediately after bubbles or abnormal pressure are detected?

First establish whether partial delivery has occurred and which container is affected. Immediate repetition may double-dose it. Act on the actual state and recheck the first cycle after recovery.

Multiport selection does not provide independent simultaneous channels. Validate washing through both liquid handling and retention of the required solid phase.

References

View references (2)
  1. [1]FOREACH ABD bubble-detection module: configurations and specifications
  2. [2]FOREACH PDM5 pressure-detection module: configurations and specifications

Related guides

  • Back to the microplate dispensing and washing fluidics overview

Related Products

ABD bubble-detection module; representative configuration. Select capacity, materials and interfaces for the application.

ABD bubble-detection module

PDM5 pressure-detection module; representative configuration. Select capacity, materials and interfaces for the application.

PDM5 pressure-detection module

Related Technical Articles

Why Do Air Bubbles Delay or Bias Piston Pump Dispensing?

A completed piston stroke does not prove that the intended liquid volume reached the vessel. Bubble compression temporarily stores displacement; expansion can then cause a trailing dispense or affect the next cycle. Locate where bubbles first appear and compare synchronized pressure and individual dispense masses before and after priming.

2026-09-10View Details

What is flow resistance calculation for? Using the Foreach calculator in fluid-path design and selection

The free flow rate of the pump meets the requirements. After installing the pipelines, valves and joints, the liquid supply at the end is insufficient. Flow resistance calculations can break the problem down into comparable data: what pressure differential is required at the target flow rate, where does the pressure loss mainly occur, and what happens if the pipe diameter, pipe length, component Cv or medium viscosity is changed. This article combines the actual function of the Foreach flow resistance calculator with a recalculable fluid circuit case to explain how to use the calculation results for design, selection, troubleshooting and prototype verification.

2026-09-11View Details

Why do multiple wash nozzles deliver different flows from one miniature diaphragm pump?

Calculate demand from the volume and timing of each wash nozzle, separate insufficient pump output from unequal distribution, and verify each branch with collection and pressure measurements.

2026-09-08View Details

Discuss a microplate workstation fluidic configuration

Provide a fluid-path diagram, actual liquids and pressures in each state. Validate detection and false alarms for the tubing, dimensions, media and action window. Confirm range and wetted configuration by model, then test normal baselines and faults separately against the guide's validation objectives.

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