What do these products do in this instrument?
Liquid diaphragm pumps: wash liquid and shared supply for microplate stations
Transfer liquid from reservoirs to actual microplate supply or wash manifolds, accounting for branch resistance, wash heads and concurrent demand. Check total supply and actual delivery to each well separately.
Compare DPL30, DPL60 and DPL30H by actual supply load. Wash-head, valve, tubing and container resistance jointly determine installed flow; check the supply window and receiving position together.
Gas-Liquid Diaphragm Pumps: Suitable Microplate Aspiration and Waste Removal
Provide suction/drainage for aspiration heads in air-containing aspiration or vessel-vacuum architectures, coordinated with wells, height and peak inflow. Confirm the configuration for the actual inlet medium and record residual volume in each well.
DPGL800 handles gas or gas–liquid aspiration permitted by its specific configuration. Check inlet gas content, foam, peak inflow and container vacuum; use a separately suitable solution for continuous liquid-only transfer.
Define liquid-supply and aerated-aspiration branches separately
Suitable liquid diaphragm-pump configurations supply wash heads, stations or working vessels. Gas–liquid diaphragm pumps serve permitted aerated aspiration or related container-vacuum architectures. Describe actual inlet medium and gas–liquid state first, then determine pump position, incoming source and discharge destination.
Match supply/removal capacity to washing-station and container conditions
Installed flow depends jointly on pump, tubing, valves, wash head and container; free flow does not directly represent station delivery. Evaluate supply by effective coverage and available time, and waste removal by peak inflow, lift, permitted residual liquid and full-container state. Check the complete wash cycle when supply and aspiration must be coordinated.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Inlet medium | Distinguish liquid-only flow, intermittent gas entrainment and foam, and confirm the aspiration modes permitted by the configuration. |
| Tubing and containers | Provide suction lift, outlet load, tubing and waste-container conditions. |
| Actual supply and removal | Measure incoming liquid, residue and emptying at the station; check handling after full level, shortage or blockage. |
| Operation | Specific operating conditions |
|---|---|
| Microplate washing and aspiration | Solid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| DPL30 liquid diaphragm pump | Liquid supply or auxiliary circulation | Select by installed resistance, actual wash or working fluid and task window |
| DPL60 liquid diaphragm pump | Liquid supply or auxiliary circulation | Judge by peak demand and installed operating point, not free flow alone |
| DPL30H high-backpressure liquid diaphragm pump | Liquid branches requiring higher backpressure capability | Calculate pressure budget and actual flow first, then confirm interfaces and materials |
| DPGL800 gas–liquid diaphragm pump | Suitable gas–liquid aspiration or container-vacuum branches | Confirm inlet medium and waste architecture; not for continuous liquid-only transfer (100% liquid) |
Explore specific operating and validation tasks
Microplate washing and aspiration
Observe residual liquid, foam and solid-phase loss during each cycle. Insufficient supply, a misaligned wash head and delayed waste removal can cause similar symptoms and must be checked separately.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Microplate washing and aspiration | Evaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery together | Insufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery |
Assess differences in delivered volume between wells, residual liquid after washing and the complete plate cycle. Preserve well identities.
Do similar nominal supply- and waste-pump flow rates make a matched pair?
Compare peak inflow, effective aspiration and permissible accumulation in the actual installation. The branches may handle different media and operating windows; validate station recovery over the complete sequence.
Multiport selection does not provide independent simultaneous channels. Validate washing through both liquid handling and retention of the required solid phase.
References
View references (4)
- [1]FOREACH DPL30 liquid diaphragm pump: configurations and specifications
- [2]FOREACH DPL60 liquid diaphragm pump: configurations and specifications
- [3]FOREACH DPL30H high-backpressure liquid diaphragm pump: configurations and specifications
- [4]FOREACH DPGL800 gas–liquid diaphragm pump: configurations and specifications