Where microplate washing and aspiration occur in the instrument
Supply, wash-head design and aspiration height jointly affect residual liquid, foam and solid-phase retention. Include incoming wash liquid and aspirated air in waste-pump operating conditions, then verify well-to-well variation across the complete plate.
Observe residual liquid, foam and solid-phase loss during each cycle. Insufficient supply, a misaligned wash head and delayed waste removal can cause similar symptoms and must be checked separately.
Product responsibilities in this operation
Diaphragm pumps perform suitable liquid supply or aerated aspiration in this task. Confirm inlet phase, branch location and outlet destination separately, then check each branch's operating point.
Compare DPL30, DPL60 and DPL30H by actual supply load. Wash-head, valve, tubing and container resistance jointly determine installed flow; check the supply window and receiving position together.
DPGL800 handles gas or gas–liquid aspiration permitted by its specific configuration. Check inlet gas content, foam, peak inflow and container vacuum; use a separately suitable solution for continuous liquid-only transfer.
For this microplate dispensing and washing task, confirm solid-phase location and retention target, wash cycles, aspiration height, residual liquid, destination and downstream method requirements.
Organize the sequence by actual architecture
- Confirm wash liquid, plate type, wash head and aspiration-tip position for the specific microplate method. Where cells, magnetic beads or other solids must be retained, identify their location and allowable loss separately.
- Supply wash liquid to the intended wells and apply contact time or mixing according to the method. Check wash-head coverage, blocked outlets and actual arrival at each well.
- Remove wash liquid at the specified aspiration height. Match waste handling to liquid-only or air-containing inlet conditions while checking foam, peak inflow, post-wash residue and waste-bottle state.
- Compare residual liquid, retained solid phase and method background across the complete plate, including first and last wells and post-maintenance recovery. Assess product recovery separately only for methods that include eluate collection.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Solid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements |
| Acceptance target | Evaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery together |
| Fault localization | Insufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| DPL30 liquid diaphragm pump | Liquid supply or auxiliary circulation | Select by installed resistance, actual wash or working fluid and task window |
| DPL60 liquid diaphragm pump | Liquid supply or auxiliary circulation | Judge by peak demand and installed operating point, not free flow alone |
| DPL30H high-backpressure liquid diaphragm pump | Liquid branches requiring higher backpressure capability | Calculate pressure budget and actual flow first, then confirm interfaces and materials |
| DPGL800 gas–liquid diaphragm pump | Suitable gas–liquid aspiration or container-vacuum branches | Confirm inlet medium and waste architecture; not for continuous liquid-only transfer (100% liquid) |
Validate operation results at the receiver
Evaluate solid-phase loss, post-wash residue and downstream assay background or nucleic-acid recovery together.
Assess differences in delivered volume between wells, residual liquid after washing and the complete plate cycle. Preserve well identities.
Locate abnormalities and restore the next operation
Insufficient capture, incorrect aspiration height, uneven coverage or pooling may impair washing and recovery.
Review actual results of affected steps before resuming the next action.
Multiport selection does not provide independent simultaneous channels. Validate washing through both liquid handling and retention of the required solid phase.
References
View references (4)
- [1]FOREACH DPL30 liquid diaphragm pump: configurations and specifications
- [2]FOREACH DPL60 liquid diaphragm pump: configurations and specifications
- [3]FOREACH DPL30H high-backpressure liquid diaphragm pump: configurations and specifications
- [4]FOREACH DPGL800 gas–liquid diaphragm pump: configurations and specifications