What do these products do in this instrument?
Tubing and fittings: connect reservoirs, manifolds and waste paths
Build supply and aspiration networks for the plate station, identifying shared sections and independent branches. Include length, bore and interface differences in per-well or per-channel delivery checks.
Tubing and fittings together form sealed connections and the actual transfer path. Check formulation, inner/outer diameters, length, tube-end positioning and port geometry, including added volume in residue and arrival delay.
Design tubing and fittings as a complete wetted path
Tubing, fittings and ports jointly determine sealing, transfer volume and medium-contact coverage. Materials and dimensions must suit formulation, operating conditions and installation. Include tubing length and interface volume in priming, shared residue and arrival delay. After connection changes, recheck delivery and cleaning through the actual path.
Calculate connection volume and post-maintenance recovery
Shared volume affects liquid arrival and flushing; interface gaps may retain the previous composition. Record length, actual bore and tube insertion position. After disassembly, reprime and check seals, then compare first-cycle delivery, blanks or recovered product to confirm path recovery.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Materials and assembly | Provide formulation, tubing inner/outer diameters, length, fitting structure and port fit. |
| Volume and load | Calculate shared and replacement-component added volumes; measure actual flow and response. |
| Maintenance recovery | Record disassembly locations and added volume; recheck priming, sealing and first-cycle received results. |
| Operation | Specific operating conditions |
|---|---|
| Manifold connections | Tubing ID/OD, length, interface geometry, pressure, materials and maintenance method |
Manifold connections
Assess flow distribution and air aspiration against branch length, bore size, fittings and manifold volume. After assembly, check sealing, blocked ports, flushing and maintenance access before measuring well-to-well performance.
After assembly, check blocked ports, leaks and path volume, and measure each well. Calibrating total volume alone can conceal local dispensing errors.
Check leaks, tube disconnection and material changes at actual pressure and temperature; evaluate residue, recovery and the first post-maintenance cycle through the complete path
Misconnections, gaps, tube deformation or leaks may alter volume and pressure. Isolate the affected segment and reconfirm assembly; matching threads do not establish interchangeability.
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| Tubing materials and dimensions | Connection paths and shared volume | Bore, length, load and actual formulation jointly determine materials and dimensions |
| Rigid-tubing fittings | Tube-end positioning and sealed connections | Check tube outer diameter, threads, seal structure and port-bottom geometry |
Related operations and architecture comparisons
Microplate washing and aspiration
Observe residual liquid, foam and solid-phase loss during each cycle. Insufficient supply, a misaligned wash head and delayed waste removal can cause similar symptoms and must be checked separately.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Manifold connections | Check leaks, tube disconnection and material changes at actual pressure and temperature; evaluate residue, recovery and the first post-maintenance cycle through the complete path | Misconnections, gaps, tube deformation or leaks may alter volume and pressure |
Assess differences in delivered volume between wells, residual liquid after washing and the complete plate cycle. Preserve well identities.
Does a fitting that tightens prove the tubing assembly is suitable?
Also confirm port geometry, tube-end positioning and seal fit. Check leakage, hold-up and performance after repeated maintenance under actual conditions; thread engagement alone is insufficient.
Multiport selection does not provide independent simultaneous channels. Validate washing through both liquid handling and retention of the required solid phase.