What do these products do in this instrument?
Bubble and pressure sensing: monitor reaction dispensing
Use the defined bubble or pressure location to observe the dispensing branch, while the controller associates the event with the active cuvette and addition phase. A signal upstream of the probe has to be interpreted with the intervening path and actual liquid-arrival sequence.
ABD provides bubble or liquid-state signals for defined tubing; PDM5 provides pressure information at a defined location. Configure module, position and action window separately; the controller associates affected samples or tasks.
Relate sensing position to the reaction-start sequence
Identify whether the monitored segment contains sample, reagent or system liquid and where it lies relative to the probe outlet. Record motor completion, liquid leaving the outlet and arrival at the reaction position as separate events when validating the sequence. Choose the observation window around the event relevant to the assay rather than around a generic motion command.
Further technical reading
Preserve timing when an addition is interrupted
An event before liquid contact and an event during or after contact leave different reaction states. Retain the cuvette, addition order, recorded times and evidence of received volume before attempting recovery. Clotting, chromogenic and immunoturbidimetric methods define different initiation or reading rules; the method decides whether a reaction can continue after the path is restored.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Observed liquid and location | Map the sample, reagent or system-liquid segment, probe outlet and sensing point so the distance between an observed event and the reaction is explicit. |
| Method timing | Define the addition phase, contact event, incubation or acquisition window and controller record used for the selected assay. |
| Interrupted reaction | Keep the affected cuvette and known or unknown delivery status with the timestamps; define the method response before allowing another addition. |
| Operation | Specific operating conditions |
|---|---|
| State monitoring | Assay-specific addition and reading sequence, sensing position, liquid-arrival observations, current cuvette and treatment of uncertain partial delivery |
State monitoring
Observe events with the actual sample and reagent sequence, including idle recovery and the first addition after washing. A signal observed during priming has a different consequence from the same signal during an assay addition.
Keep the cuvette association valid until the affected liquid could have reached its destination. Review the recorded path and timing if a delayed alarm arrives after the controller has advanced to another step.
Measure event detection and end-to-end controller response under the relevant addition phases. Compare these with actual receipt and contact observations rather than using the sensor timestamp as the reaction-start timestamp.
After restoring the path, check the next valid addition and the method response to residual wash liquid. The interrupted cuvette remains subject to its own method-defined disposition.
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| ABD bubble-detection module | Bubble or liquid-state identification in defined tubing | Validate detection and false alarms for tubing material, dimensions, medium and action window |
| PDM5 pressure-detection module | Pressure-state monitoring at a defined fluidic location | Confirm range and wetted configuration by model; validate normal baseline and abnormalities separately |
Related operations and architecture comparisons
Assay-specified sample delivery
Small sample doses require checks of tip droplets, cuvette-wall contact and the system-liquid interface. Measure at the actual reaction location to relate volume to results.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| State monitoring | Challenge the dispensing branch before, during and after the relevant addition phase. Compare sensor events and controller response with observed liquid arrival, actual receipt and retained reaction timing. | An alarm cleared after priming does not restore an earlier reaction-start time. Review the interrupted reaction separately from verification of the next valid dispensing cycle. |
Acceptance requires a useful association between the observed fluidic event and the method-defined reaction state. Verify the next addition after recovery without treating motion completion as a measurement of liquid delivery.
Can an interrupted addition resume when the bubble alarm clears?
Only the assay procedure can determine whether the recorded reaction state permits continuation. Establish whether contact or partial addition occurred, retain the original timing, and handle that cuvette separately from restoring the dispensing path.
Pump or valve response time is not the reagent-contact or instrument measurement-start time.