Where assay-specified sample delivery occurs in the instrument
Set sample type, aspiration position and residual-volume limits according to the coagulation method. Include pretreatment state, clot risk and tubing residue in delivery validation to preserve the required sample composition.
Small sample doses require checks of tip droplets, cuvette-wall contact and the system-liquid interface. Measure at the actual reaction location to relate volume to results.
Product responsibilities in this operation
Piston drive creates controlled displacement, working with valve positions and the receiver to deliver this volume. Check working stroke and actual received volume separately.
For this operation, confirm: Method-approved sample and preparation; clot risk; container and remaining volume; probe-tip position; sample/system-liquid interface; target cuvette and next reaction step.
Organize the sequence by actual architecture
- Confirm sample type, pretreatment, container and remaining volume for the current method, and identify the target reaction cuvette.
- Coordinate probe position and metering drive to sample and deliver the specified volume, recording effects of residue, droplets or obstruction on reception.
- Check actual received volume and sample composition before proceeding to assay-required preincubation or further additions; the specific method determines handling of affected cuvettes.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Method-approved sample and preparation; clot risk; container and remaining volume; probe-tip position; sample/system-liquid interface; target cuvette and next reaction step |
| Acceptance target | Measure sample reception at the reaction cuvette with the method-required sample preparation and actual probe geometry. Check low remaining volume, hanging drops, wall contact and the first transfer after idle. |
| Fault localization | An obstructed aspiration, retained droplet or unsuitable system-liquid interface can change sample volume or composition. The effect belongs to the identified cuvette and may remain after the pump completes its stroke. |
Further technical reading
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| EA standard piston pump | Finite-stroke metering | Compare configurations by routine dose, total cycle displacement and wetted materials |
| SM miniature piston pump | Discrete metering in compact installations | Check installation space first, then validate working doses and actual accuracy |
| TM ultra-miniature piston pump | Discrete metering in limited space | Check installation space and configuration before measuring actual delivery; an ultra-miniature design does not imply a smaller reliable dose |
Validate operation results at the receiver
Measure sample reception at the reaction cuvette with the method-required sample preparation and actual probe geometry. Check low remaining volume, hanging drops, wall contact and the first transfer after idle.
Assess both received volume and preserved sample composition before the method-required preincubation or next addition. Keep sampling acceptance separate from the later reagent-trigger and reading checks.
Locate abnormalities and restore the next operation
An obstructed aspiration, retained droplet or unsuitable system-liquid interface can change sample volume or composition. The effect belongs to the identified cuvette and may remain after the pump completes its stroke.
Pause the affected reaction, identify the sample and receiving cuvette, and establish whether delivery was absent, partial or complete. Correct the sampling fault and handle the cuvette according to the method before repeating or continuing the sequence.
The coagulation method defines sample suitability and the disposition of an interrupted reaction. A metering specification does not replace sampling and complete-method validation.