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Home/Applications/In Vitro Diagnostics/Hematology analyzer
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Piston pumps in hematology analyzers

Application guide

Focusing on whole-blood sampling and reagent metering in hematology analyzers, this page explains piston-pump location, coordination and configuration conditions, linking to related operating and validation tasks.

Applicable architecture

Hematology analyzers may use different detection principles and channel configurations. Diluent, lysing reagent and sheath-fluid locations depend on the specific method; sheath-flow conditions do not apply to every counting channel.[4]

Application guide

Hematology analyzer

Hematology analyzer
Diaphragm pumps in hematology analyzers
Hematology-channel cleaning and waste removal
Bubble and pressure sensing in hematology analyzers
Tubing and fittings in hematology analyzers
Multiport rotary valves in hematology analyzers
Detection-channel switching and fluid renewal
Piston pumps in hematology analyzers
Whole-blood sampling and delivery
Sampling probes in hematology analyzers
Syringe pumps in hematology analyzers
Diluent and reaction-reagent metering

On this page

What do these products do in this instrument?Distinguish piston displacement from actual received volumeCombine metering, valve positions and container actions into a complete cycleTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageExplore specific operating and validation tasksConfirm product roles in actual operationAre piston pumps of equal capacity directly interchangeable?ReferencesRelated guides

01What do these products do in this instrument?

Piston pumps: whole-blood sampling and reagent metering

Drive sampling or specified reagent volumes in suitable metering architectures, coordinated with sampling probes, sample mixing and low remaining liquid in the source container. Validate sample volume and subsequent dilution ratio separately.

EA, SM and TM provide options for comparing capacity, wetted configurations and installation space. Verify actual doses from working stroke and the complete wetted path; check system-liquid drive and direct sample contact with the pump head separately.

02Distinguish piston displacement from actual received volume

Piston pumps use controlled displacement for discrete aspiration and dispensing. Sample may enter the head directly or remain at the probe tip while system liquid transmits displacement; wetted coverage, priming and cleaning differ. Map aspiration, holding, dispensing and replenishment paths first, then relate stroke to probe-tip delivery.

Further technical reading

  • What Is a Precision Piston Pump? Working Principle, Benefits and Applications
  • Piston Pump Accuracy, Repeatability and Resolution Explained

03Combine metering, valve positions and container actions into a complete cycle

Delivery requires coordination of metering drive, source switching and receiving-end actions. Usable capacity must cover working volume and other cycle displacements, with replenishment in permitted windows. Check tip droplets, bubbles and shared residual liquid through the complete path; remeasure received volume after changing pump heads, tubing lengths or interfaces.

04Translate actual branch conditions into configuration requirements

Piston-pump configuration conditions in hematology analyzers
ConditionTo confirm
Working strokeDefine the whole-blood sampling dose and any reagent task separately, accounting for the usable stroke and replenishment needed by the actual architecture.
Liquid contact and pressureMap the blood-contact or system-liquid path, probe position and pressure states together with sample mixing and the source volume remaining.
Delivery and recoveryCheck repeated sampling with actual whole-blood conditions, low source volume and probe washing. Verify the delivered sample volume separately from the later dilution ratio.
Operations supported in this instrument
OperationSpecific operating conditions
Whole-blood sampling and deliveryWhole-blood condition and mixing, sampling dose, time between repeated aspirations, remaining source volume, probe position and actual metering architecture

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
EA standard piston pumpFinite-stroke meteringCompare configurations by routine dose, total cycle displacement and wetted materials
SM miniature piston pumpDiscrete metering in compact installationsCheck installation space first, then validate working doses and actual accuracy
TM ultra-miniature piston pumpDiscrete metering in limited spaceCheck installation space and configuration before measuring actual delivery; an ultra-miniature design does not imply a smaller reliable dose
  • EA standard piston pump

    EA standard piston pump

    View Details
  • SM miniature piston pump

    SM miniature piston pump

    View Details
  • TM ultra-miniature piston pump

    TM ultra-miniature piston pump

    View Details

06Explore specific operating and validation tasks

Whole-blood sampling and delivery

Sample condition may change between repeated aspirations. Compare containers with normal remaining sample volume, low remaining sample volume and particle risks, recording tip position and air-aspiration handling rather than testing only repeated water aspiration/dispensing.

  • Whole-blood sampling and delivery

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Whole-blood sampling and deliveryCompare received sample volumes through repeated whole-blood sampling at normal and low source levels. Check sample changes and wash recovery, then validate the subsequent dilution ratio separately.Changes in sample condition, particles or clots, air aspiration, probe-wall contact or leakage can affect sampling. Use the actual sample and channel state when investigating an abnormal result.

Assess whole-blood sampling and recovery across the complete sample sequence. Metered sample volume, subsequent dilution ratio and detection-channel recovery are separate acceptance results.

08Are piston pumps of equal capacity directly interchangeable?

Also check wetted materials, interfaces, mounting, drive and pressure conditions. After replacement, recheck delivery, cleaning and first-dose recovery with the original sequence and actual liquids. Capacity is only one comparison criterion.

Metered dilution and sheath-fluid flow are different roles; sheath-fluid requirements depend on the detection architecture.

References

View references (4)
  1. [1]FOREACH EA standard piston pump: configurations and specifications
  2. [2]FOREACH SM miniature piston pump: configurations and specifications
  3. [3]FOREACH TM ultra-miniature piston pump: configurations and specifications
  4. [4]Sysmex hematology analyzer reference: impedance and fluorescence flow-cytometry configurations

Related guides

  • Back to the hematology analyzer fluidics overview
  • Whole-blood sampling and delivery

Related Products

EA Standard piston pumps

EA Standard piston pumps

SM Miniature piston pumps

SM Miniature piston pumps

TM Ultra-compact piston pumps

TM Ultra-compact piston pumps

Related Technical Articles

What Is a Precision Piston Pump? Working Principle, Benefits and Applications

Learn how precision piston pumps work, where they are used and how to select capacity, performance, materials, valves and controls for an OEM fluidic system.

2026-06-19View Details

Piston Pump Accuracy, Repeatability and Resolution Explained

Learn why theoretical volume per step is not the minimum reliable dose, and how accuracy, repeatability, valves, bubbles and working stroke affect dispensing results.

2026-09-06View Details

Discuss Your IVD Fluidic Requirements

Share the instrument type, sample and reagent properties, working volumes, cycle timing, fluid path and acceptance criteria so we can discuss suitable components and prototype evaluation.

Discuss your application