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Home/Applications/In Vitro Diagnostics/Hematology analyzer
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Sampling probes in hematology analyzers

Application guide

Focusing on the whole-blood aspiration interface in hematology analyzers, this page explains sampling-probe location, coordination and configuration conditions, linking to related operating and validation tasks.

Applicable architecture

Hematology analyzers may use different detection principles and channel configurations. Diluent, lysing reagent and sheath-fluid locations depend on the specific method; sheath-flow conditions do not apply to every counting channel.[2]

Application guide

Hematology analyzer

Hematology analyzer
Diaphragm pumps in hematology analyzers
Hematology-channel cleaning and waste removal
Bubble and pressure sensing in hematology analyzers
Tubing and fittings in hematology analyzers
Multiport rotary valves in hematology analyzers
Detection-channel switching and fluid renewal
Piston pumps in hematology analyzers
Whole-blood sampling and delivery
Sampling probes in hematology analyzers
Syringe pumps in hematology analyzers
Diluent and reaction-reagent metering

On this page

What do these products do in this instrument?Match component geometry to actual containersEvaluate interfaces by receiving or cleaning resultsTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageRelated operations and architecture comparisonsConfirm product roles in actual operationCan sampling and wash probes be replaced based on similar appearance alone?ReferencesRelated guides

01What do these products do in this instrument?

Sampling probes: whole-blood aspiration interface

Establish the sampling position inside the container, coordinated with mixing, metering drive and level control. Check wall contact, low remaining sample volume in the container and particle risks with the complete sampling/cleaning sequence.

Custom sampling probes connect source containers to receiving positions. Diameter, tip, surface and interface depend on sample and container. Tip geometry must work with metering drive and motion sequences.

02Match component geometry to actual containers

Sampling probes must fit container openings, bottoms, minimum levels and motion strokes. Record actual contact surfaces and permitted positions before defining tips, interfaces and motion space; appearance alone is insufficient.

Further technical reading

  • Piston Pump Accuracy, Repeatability and Resolution Explained
  • Why Do Air Bubbles Delay or Bias Piston Pump Dispensing?

03Evaluate interfaces by receiving or cleaning results

At the source, check reachable positions and low remaining volume; at the receiver, check actual volume, droplets or permitted residue. Test wetted-surface maintenance and recovery for the next container during consecutive operation. Reconfirm position and delivery after changing probes, tubing length or containers.

04Translate actual branch conditions into configuration requirements

Sampling-probe configuration conditions in hematology analyzers
ConditionTo confirm
Container and positionProvide opening, bottom, minimum level, positioning error and available motion space.
Liquid contact and surfacesSpecify sample or reagent, residue requirements and internal/external probe cleaning.
Actual functionCheck delivery, coverage, residue, solid-phase retention or mixing results according to the task.
Operations supported in this instrument
OperationSpecific operating conditions
Whole-blood sampling and deliveryActual sample volume, remaining source volume, probe-tip height, aspiration/dispensing speed and system liquid
Hematology-channel cleaning and waste removalWash-fluid formulation, volume/time, spray/aspiration geometry, air/foam and waste capacity

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
Custom sampling probeSampling interface between source container and fluidic pathCompare custom geometry by minimum liquid level, probe-tip clearance and actual sample
  • Custom sampling probe

    Custom sampling probe

    View Details

06Related operations and architecture comparisons

Whole-blood sampling and delivery

Sample condition may change between repeated aspirations. Compare containers with normal remaining sample volume, low remaining sample volume and particle risks, recording tip position and air-aspiration handling rather than testing only repeated water aspiration/dispensing.

  • Whole-blood sampling and delivery

Hematology-channel cleaning and waste removal

Cleaning must cover sampling, reaction and detection branches; waste removal must handle simultaneous inflow. After restart, check blanks and channel state before resuming sample measurement.

  • Hematology-channel cleaning and waste removal

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Whole-blood sampling and deliveryMeasure actual delivery at the receiver, covering routine small volumes and maximum task volume, first dose after idle, sample changes and high/low concentration sequencesAir aspiration, clots/particles, probe contact with a wall or leakage alter actual delivery
Hematology-channel cleaning and waste removalConfirm supply and peak waste-removal capacity under installed resistance; measure residual liquid, carryover or assay-specified recoveryInsufficient supply, foam, dry aspiration and a full waste container disrupt the cleaning cycle

Check sampling, ratios and channel recovery with actual whole-blood conditions, and validate supply stability during continuous testing.

08Can sampling and wash probes be replaced based on similar appearance alone?

Confirm tip, channels, interfaces and operating positions separately, then validate each probe's aspiration/dispensing or cleaning results. Similar appearance does not imply equal wetted volume, coverage or residual-liquid levels.

Metered dilution and sheath-fluid flow are different roles; sheath-fluid requirements depend on the detection architecture.

References

View references (2)
  1. [1]FOREACH custom sampling probes: configurations and specifications
  2. [2]Sysmex hematology analyzer reference: impedance and fluorescence flow-cytometry configurations

Related guides

  • Back to the hematology analyzer fluidics overview

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Sampling probes

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Discuss Your IVD Fluidic Requirements

Share the instrument type, sample and reagent properties, working volumes, cycle timing, fluid path and acceptance criteria so we can discuss suitable components and prototype evaluation.

Discuss your application