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Home/Applications/In Vitro Diagnostics/Hematology analyzer
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Tubing and fittings in hematology analyzers

Application guide

Hematology fluid paths connect blood sampling, dilution, reagent treatment and detection. Choose tubing and fittings by the medium and role of each branch, then verify how a changed connection affects sample delivery, channel renewal and the first measurement after maintenance.

Applicable architecture

Hematology analyzers may use different detection principles and channel configurations. Diluent, lysing reagent and sheath-fluid locations depend on the specific method; sheath-flow conditions do not apply to every counting channel.[3]

Application guide

Hematology analyzer

Hematology analyzer
Diaphragm pumps in hematology analyzers
Hematology-channel cleaning and waste removal
Bubble and pressure sensing in hematology analyzers
Tubing and fittings in hematology analyzers
Multiport rotary valves in hematology analyzers
Detection-channel switching and fluid renewal
Piston pumps in hematology analyzers
Whole-blood sampling and delivery
Sampling probes in hematology analyzers
Syringe pumps in hematology analyzers
Diluent and reaction-reagent metering

On this page

What do these products do in this instrument?Choose dimensions by the material moving through the branchRestore channel state after opening the circuitTranslate actual branch conditions into configuration requirementsCompare product configurations by the roles on this pageRelated operations and architecture comparisonsConfirm product roles in actual operationCan a blood-sampling tube be replaced with a similar tube from a reagent branch?ReferencesRelated guides

01What do these products do in this instrument?

Tubing and fittings: connect sampling, dilution and detection branches

Form the real blood-contact, reagent and detection-supply paths. A blood-sampling line, a metered diluent line and a method-required sheath branch do not have interchangeable flow or renewal duties, even when their fittings look similar.

Tubing and fittings together form sealed connections and the actual transfer path. Check formulation, inner/outer diameters, length, tube-end positioning and port geometry, including added volume in residue and arrival delay.

02Choose dimensions by the material moving through the branch

For the sample path, include mixed whole blood, remaining specimen volume and the actual pressure states in the bore and routing decision. Reagent paths must suit their formulations and cleaning exposure. Avoid selecting every branch from one nominal tube size: reducing held volume and providing the required installed flow are competing considerations that must be resolved for that branch.

Further technical reading

  • Microfluidic Hard-Tube Fitting Selection Guide: Flanged, Ferrule and Snap-Ring Connections
  • Why Does Actual Flow Change So Much When the Tubing Size Changes on the Same Miniature Diaphragm Pump?
  • What is flow resistance calculation for? Using the Foreach calculator in fluid-path design and selection

03Restore channel state after opening the circuit

Replacement introduces a new assembly state and may change held volume or admit air. Establish the correct liquid path, prime toward its intended destination and check sample renewal before measurement. Compare the channel blank and method-required detection conditions after cleaning; restoring a sampled volume alone does not show that the downstream channel has recovered.

04Translate actual branch conditions into configuration requirements

Tubing and fitting configuration conditions in hematology analyzers
ConditionTo confirm
Blood-contact sectionRecord actual sample handling, contact surfaces, bore, routing and pressure states, including the wash procedure for that section.
Reagent and detection sectionsSeparate metered diluent and lysing-reagent duties from any required continuous supply; identify the relevant channel rather than assuming a common sheath architecture.
Maintenance releaseDocument replaced dimensions and seating, priming destination, channel renewal and the blank or recovery result before processing samples.
Operations supported in this instrument
OperationSpecific operating conditions
Whole-blood sampling and deliveryBlood-contact or system-liquid sampling path, sample mixing and remaining volume, installed connection dimensions and probe wash sequence
Hematology-channel cleaning and waste removalSampling, reaction and detection segments reached by cleaning; actual return/waste routing, air removal and the channel blank required after service

05Compare product configurations by the roles on this page

Configuration options for the product roles on this page
ProductConfiguration optionConditions to verify
Tubing materials and dimensionsConnection paths and shared volumeBore, length, load and actual formulation jointly determine materials and dimensions
Rigid-tubing fittingsTube-end positioning and sealed connectionsCheck tube outer diameter, threads, seal structure and port-bottom geometry
  • Tubing materials and dimensions

    Tubing materials and dimensions

    View Details
  • Rigid-tubing fittings

    Rigid-tubing fittings

    View Details

06Related operations and architecture comparisons

Whole-blood sampling and delivery

Sample condition may change between repeated aspirations. Compare containers with normal remaining sample volume, low remaining sample volume and particle risks, recording tip position and air-aspiration handling rather than testing only repeated water aspiration/dispensing.

  • Whole-blood sampling and delivery

Hematology-channel cleaning and waste removal

Cleaning must cover sampling, reaction and detection branches; waste removal must handle simultaneous inflow. After restart, check blanks and channel state before resuming sample measurement.

  • Hematology-channel cleaning and waste removal

07Confirm product roles in actual operation

Operating-point validation and fault localization
OperationMeasurements and observationsPriority checks
Whole-blood sampling and deliveryMeasure whole-blood sample receipt through the serviced path using representative permitted conditions, then verify the subsequent dilution separately.An altered bore, deformation or trapped gas may change aspiration; a correct sample dose does not establish the prepared dilution.
Hematology-channel cleaning and waste removalCheck the channel renewal sequence, air clearance and blank after cleaning or replacement, followed by the method-required recovery measurement.A path can be leak-tight but retain old sample or cleaning liquid. Investigate the affected segment and channel state before repeating measurement.

Release the serviced circuit with separate evidence for sample transfer, preparation and channel recovery. Preserve its routing and installed dimensions so future replacements reproduce the validated arrangement.

08Can a blood-sampling tube be replaced with a similar tube from a reagent branch?

Compare the actual bore, materials, pressure states and cleaning duty first. A reagent branch may not share the same sample-contact or renewal requirements; verify blood transfer and downstream recovery in the complete assembly.

Metered dilution and sheath-fluid flow are different roles; sheath-fluid requirements depend on the detection architecture.

References

View references (3)
  1. [1]FOREACH tubing materials and dimensions: configurations and specifications
  2. [2]FOREACH rigid-tubing fittings: configurations and specifications
  3. [3]Sysmex hematology analyzer reference: impedance and fluorescence flow-cytometry configurations

Related guides

  • Back to the hematology analyzer fluidics overview

Related Products

Tubing

Tubing

Hard-tube fittings

Hard-tube fittings

Related Technical Articles

Microfluidic Hard-Tube Fitting Selection Guide: Flanged, Ferrule and Snap-Ring Connections

Selection essentials: Check port geometry, tubing OD and fluid first, then pressure, maintenance frequency and tube-end preparation. Flanged fittings press a tube-end flange against a flat sealing face; ferrules grip the tube as the fitting tightens; snap-ring designs use a sleeve and sealing ring. Matching threads and tube diameters alone do not establish compatibility.

2026-10-12View Details

Why Does Actual Flow Change So Much When the Tubing Size Changes on the Same Miniature Diaphragm Pump?

Even when the pump is unchanged, installed flow can change because of tubing inner diameter, length, bends, and the minimum bore of fittings. When flow falls after a tubing change, the effective response is neither to blame the pump first nor to enlarge every tube indiscriminately. Measure inlet pressure, outlet pressure, and accumulated flow together to determine where the added restriction actually occurs.

2026-08-24View Details

What is flow resistance calculation for? Using the Foreach calculator in fluid-path design and selection

The free flow rate of the pump meets the requirements. After installing the pipelines, valves and joints, the liquid supply at the end is insufficient. Flow resistance calculations can break the problem down into comparable data: what pressure differential is required at the target flow rate, where does the pressure loss mainly occur, and what happens if the pipe diameter, pipe length, component Cv or medium viscosity is changed. This article combines the actual function of the Foreach flow resistance calculator with a recalculable fluid circuit case to explain how to use the calculation results for design, selection, troubleshooting and prototype verification.

2026-09-11View Details

Discuss Your IVD Fluidic Requirements

Share the instrument type, sample and reagent properties, working volumes, cycle timing, fluid path and acceptance criteria so we can discuss suitable components and prototype evaluation.

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