What do these products do in this instrument?
Syringe pumps: diluent and sample transfer
Organize sample transfer or diluent delivery in syringe-driven branches, using valves and reaction positions to prepare the required ratio. If the method uses lysing reagent, check its actual dose separately.
HLD and HMD organize aspiration/dispensing paths with rotary and solenoid valves respectively. Capacity budgets include useful volume, holding stages and replenishment points; valve states and pump actions together create each delivery.
Organize syringe metering around aspiration, holding and dispensing
Syringe drives use controlled displacement for aspiration, holding and dispensing. With direct contact, source liquid enters the metering chamber; with system-liquid drive, sample can remain at the probe tip or a designated tube segment. Define wetted coverage before capacity, cleaning and replenishment. For repeated dispensing, track current inventory, delivered volume and next replenishment time.
Further technical reading
Map valve connections to every action
Check port connections, opening/closing sequence and power-off state separately for rotary- and solenoid-valve configurations. Changing source does not automatically remove old liquid from the chamber or shared tubing. Plan flushing for formulation and residue requirements, then evaluate the first dose after switching and dose-to-dose variation.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Stored volume and timing | Define volume per operation, consecutive dispensing count, holding time and permitted replenishment window. |
| Valve positions and destinations | Provide a source, receiver and waste port diagram with open/closed and power-off states. |
| Liquid changes and first dose | Check shared residue, priming destination and actual received volume after standby or source changes. |
| Operation | Specific operating conditions |
|---|---|
| Diluent and reaction-reagent metering | Actual volumes of original sample, diluent or internal standard; concentrations at each stage; mixing and resampling conditions |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| HLD rotary-valve syringe pump | Aspiration, holding and dispensing with a rotary valve | Check source/outlet valve positions, capacity budget and replenishment timing |
| HMD solenoid-valve syringe pump | Metered aspiration and dispensing with solenoid valves | Check open/closed and power-off states, working volume and replenishment cycle |
Explore specific operating and validation tasks
Diluent and reaction-reagent metering
Equal commanded volumes in two branches do not establish the correct final ratio. Check delivery errors, tubing residue and mixing together within the assay-defined dilution step.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Diluent and reaction-reagent metering | Measure each branch's delivery, received ratio and mixing uniformity separately, then check assay response | Small-volume errors, shared residual liquid or inadequate mixing may alter the actual ratio |
Check sampling, ratios and channel recovery with actual whole-blood conditions, and validate supply stability during continuous testing.
For repeated dispensing from one chamber, is checking total volume enough?
Measure each received dose, especially the first and last. Even when total volume is near target, individual errors may differ. Relate replenishment, valve switching and receiving actions to each result.
Metered dilution and sheath-fluid flow are different roles; sheath-fluid requirements depend on the detection architecture.