Where whole-blood sampling and delivery occur in the instrument
Confirm whole-blood mixing and container conditions before setting aspiration height, speed and remaining-volume limits. Consider clots, wall contact, viscosity changes and post-sampling flushing; water repeatability does not establish whole-blood performance.
Sample condition may change between repeated aspirations. Compare containers with normal remaining sample volume, low remaining sample volume and particle risks, recording tip position and air-aspiration handling rather than testing only repeated water aspiration/dispensing.
Product responsibilities in this operation
Piston drive creates controlled displacement, working with valve positions and the receiver to deliver this volume. Check working stroke and actual received volume separately.
For this operation, confirm: Whole-blood identity and mixing state; container geometry and remaining volume; clot or particle risk; probe position; aspiration program; sample receiver and subsequent dilution.
Organize the sequence by actual architecture
- Confirm whole-blood sample identity, mixing state, container and remaining volume, then set suitable probe-tip position and aspiration sequence.
- Sample with the metering drive while observing air aspiration, wall contact or obstruction; record tip and receiver delivery under actual whole-blood conditions.
- Deliver the collected sample to its specified reaction path, validating sample volume and subsequent dilution ratio separately. After cleaning, check blanks and recovery for the next sample.
Define operating conditions for this task
| To define | Details |
|---|---|
| Actual operating conditions | Whole-blood identity and mixing state; container geometry and remaining volume; clot or particle risk; probe position; aspiration program; sample receiver and subsequent dilution |
| Acceptance target | Measure whole-blood delivery at the reaction receiver after the specified mixing procedure. Cover normal and low source volumes and repeated aspirations, recording probe position, air aspiration and obstruction separately. |
| Fault localization | Settling, clots, wall contact or incomplete aspiration can change the amount or composition of blood delivered. A correct commanded stroke cannot establish that a representative aliquot entered the reaction path. |
Further technical reading
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| EA standard piston pump | Finite-stroke metering | Compare configurations by routine dose, total cycle displacement and wetted materials |
| SM miniature piston pump | Discrete metering in compact installations | Check installation space first, then validate working doses and actual accuracy |
| TM ultra-miniature piston pump | Discrete metering in limited space | Check installation space and configuration before measuring actual delivery; an ultra-miniature design does not imply a smaller reliable dose |
Validate operation results at the receiver
Measure whole-blood delivery at the reaction receiver after the specified mixing procedure. Cover normal and low source volumes and repeated aspirations, recording probe position, air aspiration and obstruction separately.
Verify sample volume before assessing the subsequent dilution ratio and channel result. After washing, check next-sample blanks and recovery so changes in blood condition are not hidden by water-only repeatability tests.
Locate abnormalities and restore the next operation
Settling, clots, wall contact or incomplete aspiration can change the amount or composition of blood delivered. A correct commanded stroke cannot establish that a representative aliquot entered the reaction path.
Hold the affected specimen and reaction position, review the last mixing and aspiration state, and establish delivered volume. Address the obstruction or supply fault and follow the method for resampling or rejecting the affected aliquot before checking the next sample.
Whole-blood delivery, dilution and detection-channel performance require separate evidence. Results from repeated water transfers do not establish whole-blood sampling performance.
References
View references (4)
- [1]FOREACH EA standard piston pump: configurations and specifications
- [2]FOREACH SM miniature piston pump: configurations and specifications
- [3]FOREACH TM ultra-miniature piston pump: configurations and specifications
- [4]Sysmex hematology analyzer reference: impedance and fluorescence flow-cytometry configurations