What do these products do in this instrument?
Liquid diaphragm pumps: magnetic-separation and probe-wash supply
Supply wash fluid to assay-required wash heads or probe stations. In magnetic solid-phase architectures, coordinate magnetic capture, wash-head position and aspiration branches; fluid supply itself does not perform magnetic separation.
Compare DPL30, DPL60 and DPL30H by actual supply load. Wash-head, valve, tubing and container resistance jointly determine installed flow; check the supply window and receiving position together.
Gas–liquid diaphragm pumps: aerated waste removal at washing stations
Aspirate residual wash liquid through waste paths containing air or gas–liquid mixtures, or serve suitable waste-container vacuum branches. Coordinate aspiration probes, peak supply and full-level interlocks to prevent pooling from affecting the next wash.
DPGL800 handles gas or gas–liquid aspiration permitted by its specific configuration. Check inlet gas content, foam, peak inflow and container vacuum; use a separately suitable solution for continuous liquid-only transfer.
Define liquid-supply and aerated-aspiration branches separately
Suitable liquid diaphragm-pump configurations supply wash heads, stations or working vessels. Gas–liquid diaphragm pumps serve permitted aerated aspiration or related container-vacuum architectures. Describe actual inlet medium and gas–liquid state first, then determine pump position, incoming source and discharge destination.
Further technical reading
Match supply/removal capacity to washing-station and container conditions
Installed flow depends jointly on pump, tubing, valves, wash head and container; free flow does not directly represent station delivery. Evaluate supply by effective coverage and available time, and waste removal by peak inflow, lift, permitted residual liquid and full-container state. Check the complete wash cycle when supply and aspiration must be coordinated.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Inlet medium | Distinguish liquid-only flow, intermittent gas entrainment and foam, and confirm the aspiration modes permitted by the configuration. |
| Tubing and containers | Provide suction lift, outlet load, tubing and waste-container conditions. |
| Actual supply and removal | Measure incoming liquid, residue and emptying at the station; check handling after full level, shortage or blockage. |
| Operation | Specific operating conditions |
|---|---|
| Fluid supply and waste removal for magnetic solid-phase washing | Solid-phase location and retention target, wash cycles, aspiration height, residue, destination and downstream assay requirements |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| DPL30 liquid diaphragm pump | Liquid supply or auxiliary circulation | Select by installed resistance, actual wash or working fluid and task window |
| DPL60 liquid diaphragm pump | Liquid supply or auxiliary circulation | Judge by peak demand and installed operating point, not free flow alone |
| DPL30H high-backpressure liquid diaphragm pump | Liquid branches requiring higher backpressure capability | Calculate pressure budget and actual flow first, then confirm interfaces and materials |
| DPGL800 gas–liquid diaphragm pump | Suitable gas–liquid aspiration or container-vacuum branches | Confirm inlet medium and waste architecture; not for continuous liquid-only transfer (100% liquid) |
Explore specific operating and validation tasks
Fluid supply and waste removal for magnetic solid-phase washing
Record added volume, residual liquid and bead loss in every wash. If background is abnormal, distinguish inadequate capture, coverage, aspiration positioning and waste pooling before changing pump output.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Fluid supply and waste removal for magnetic solid-phase washing | Measure bead loss, post-wash residue and downstream assay background for each wash; do not evaluate washing by volume or recovery alone | Insufficient magnetic capture, misplaced aspiration, wash coverage or waste pooling may increase background |
Evaluate reagent delivery, post-wash residue, bead loss and assay background together.
Do similar nominal supply- and waste-pump flow rates make a matched pair?
Compare peak inflow, effective aspiration and permissible accumulation in the actual installation. The branches may handle different media and operating windows; validate station recovery over the complete sequence.
Pump flow alone does not prove adequate washing; validate magnetic capture, mixing and aspiration position across the whole station.
References
View references (5)
- [1]FOREACH DPL30 liquid diaphragm pump: configurations and specifications
- [2]FOREACH DPL60 liquid diaphragm pump: configurations and specifications
- [3]FOREACH DPL30H high-backpressure liquid diaphragm pump: configurations and specifications
- [4]FOREACH DPGL800 gas–liquid diaphragm pump: configurations and specifications
- [5]Beckman Coulter Access: an assay example of magnetic solid-phase separation, washing and substrate detection